State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P. R. China.
Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, P. R. China.
Langmuir. 2023 Mar 7;39(9):3216-3224. doi: 10.1021/acs.langmuir.2c02909. Epub 2023 Feb 23.
In this work, we synthesize a series of hierarchically organized gold nanoparticles (Au HOPs-X) with peroxidase (POD)-like catalytic activity by the in situ reduction of Au-thiolate hierarchically organized particles (Au HOPs). The initial Au HOPs show little POD-like catalytic activity. However, after the reduction of the particles, the Au HOPs-X showed enhanced POD-like catalytic activity, where X represents the reduction degree of Au HOPs. The reasons are as follows: (1) the Au-thiolate complexes on the surface of the Au HOPs-X were reduced into Au nanoparticles, and the active Au content increases with the increase of the reduction degree; (2) the specific surface area of Au HOPs-X becomes larger. Based on this, the Au HOPs-10 with the highest catalytic activity were combined with glucose oxidase to obtain a standard curve as a function of glucose concentrations. The color of the solutions was captured by mobile phone photos to determine their saturation, and the rapid detection of glucose was achieved through the standard curve of glucose concentration and saturation determined in this study.
在这项工作中，我们通过金-硫醇盐分级组装粒子（Au HOPs）的原位还原，合成了一系列具有过氧化物酶（POD）样催化活性的分级组装金纳米粒子（Au HOPs-X）。初始的 Au HOPs 表现出很少的 POD 样催化活性。然而，在颗粒还原后，Au HOPs-X 表现出增强的 POD 样催化活性，其中 X 代表 Au HOPs 的还原程度。原因如下：（1）Au HOPs-X 表面的 Au-硫醇盐复合物被还原成金纳米粒子，并且随着还原程度的增加，活性 Au 含量增加；（2）Au HOPs-X 的比表面积增大。基于此，将具有最高催化活性的 Au HOPs-10 与葡萄糖氧化酶结合，得到了一个作为葡萄糖浓度函数的标准曲线。通过手机照片捕捉溶液的颜色来确定其饱和度，并通过本研究确定的葡萄糖浓度和饱和度标准曲线实现了对葡萄糖的快速检测。
