Department of Biochemistry, Purdue University, West Lafayette, IN, United States; Center for Plant Biology, Purdue University, West Lafayette, IN, United States.
Department of Biochemistry, Purdue University, West Lafayette, IN, United States.
Plant Sci. 2023 May;330:111662. doi: 10.1016/j.plantsci.2023.111662. Epub 2023 Feb 22.
Plants appear quiet: quietly, they break the ground, expand leaves, search for resources, alert each other to invaders, and heal their own wounds. In contrast to the stationary appearance, the inside world of a plant is full of movements: cells divide to increase the body mass and form new organs; signaling molecules migrate among cells and tissues to drive transcriptional cascades and developmental programs; macromolecules, such as RNAs and proteins, collaborate with different partners to maintain optimal organismal function under changing cellular and environmental conditions. All these activities require a dynamic yet appropriately controlled molecular network in plant cells. In this short review, we used the regulation of cullin-RING ubiquitin ligases (CRLs) as an example to discuss how dynamic biochemical processes contribute to plant development. CRLs comprise a large family of modular multi-unit enzymes that determine the activity and stability of diverse regulatory proteins playing crucial roles in plant growth and development. The mechanism governing the dynamic assembly of CRLs is essential for CRL activity and biological function, and it may provide insights and implications for the regulation of other dynamic multi-unit complexes involved in fundamental processes such as transcription, translation, and protein sorting in plants.
它们悄然破土而出，展开叶片，寻找资源，互相提醒有外来者入侵，还能自我疗伤。与植物静止的外表形成鲜明对比的是，植物的内部世界充满了各种活动：细胞分裂以增加体量并形成新的器官；信号分子在细胞和组织间迁移，驱动转录级联和发育程序；大分子，如 RNA 和蛋白质，与不同的伙伴协作，在细胞和环境条件不断变化的情况下维持最佳的整体功能。所有这些活动都需要植物细胞中一个动态但又受到适当控制的分子网络。在这篇短评中，我们以 Cullin-RING 泛素连接酶 (CRLs) 的调控为例，讨论了动态生化过程如何促进植物发育。CRLs 是一大类模块化多单位酶，决定了多种调控蛋白的活性和稳定性，这些蛋白在植物生长和发育中起着至关重要的作用。调控 CRL 动态组装的机制对于 CRL 的活性和生物学功能至关重要，它可能为其他动态多单位复合物的调控提供启示和影响，这些复合物参与了植物中基本过程的调控，如转录、翻译和蛋白质分拣。
