• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过血管加压素受体 V1a2 的基因编辑培育攻击性较弱的幼鲐。

Development of a chub mackerel with less-aggressive fry stage by genome editing of arginine vasotocin receptor V1a2.

机构信息

Aqua-Bioresource Innovation Center (ABRIC) Karatsu Satellite, Faculty of Agriculture, Kyushu University, Saga, 847-0132, Japan.

Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka, 819-0395, Japan.

出版信息

Sci Rep. 2023 Feb 23;13(1):3190. doi: 10.1038/s41598-023-30259-x.

DOI:10.1038/s41598-023-30259-x
PMID:36823281
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9950132/
Abstract

Genome editing is a technology that can remarkably accelerate crop and animal breeding via artificial induction of desired traits with high accuracy. This study aimed to develop a chub mackerel variety with reduced aggression using an experimental system that enables efficient egg collection and genome editing. Sexual maturation and control of spawning season and time were technologically facilitated by controlling the photoperiod and water temperature of the rearing tank. In addition, appropriate low-temperature treatment conditions for delaying cleavage, shape of the glass capillary, and injection site were examined in detail in order to develop an efficient and robust microinjection system for the study. An arginine vasotocin receptor V1a2 (V1a2) knockout (KO) strain of chub mackerel was developed in order to reduce the frequency of cannibalistic behavior at the fry stage. Video data analysis using bioimage informatics quantified the frequency of aggressive behavior, indicating a significant 46% reduction (P = 0.0229) in the frequency of cannibalistic behavior than in wild type. Furthermore, in the V1a2 KO strain, the frequency of collisions with the wall and oxygen consumption also decreased. Overall, the manageable and calm phenotype reported here can potentially contribute to the development of a stable and sustainable marine product.

摘要

基因组编辑是一种通过人工诱导所需性状的高精度来显著加速作物和动物育种的技术。本研究旨在利用一种能够高效收集卵子和进行基因组编辑的实验系统，开发一种具有低攻击性的竹荚鱼品种。通过控制养殖池的光周期和水温，实现了性成熟和控制产卵季节和时间的技术辅助。此外，还详细检查了合适的低温处理条件、玻璃毛细管的形状和注射部位，以开发一种高效、稳健的微注射系统。为了减少幼鱼阶段的同类相食行为，开发了一种竹荚鱼血管加压素受体 V1a2（V1a2）敲除（KO）品系。使用生物图像信息学对视频数据进行分析，定量分析了攻击性行为的频率，表明与野生型相比，竹荚鱼的同类相食行为频率显著降低了 46%（P=0.0229）。此外，在 V1a2 KO 品系中，与墙壁碰撞的频率和耗氧量也降低了。总的来说，这里报道的可管理和冷静的表型可能有助于开发稳定和可持续的海洋产品。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/9f01b0646721/41598_2023_30259_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/adeadd75d63d/41598_2023_30259_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/800a93cc32c1/41598_2023_30259_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/2d9536e93bda/41598_2023_30259_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/bd482d3d1bcf/41598_2023_30259_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/9f01b0646721/41598_2023_30259_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/adeadd75d63d/41598_2023_30259_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/800a93cc32c1/41598_2023_30259_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/2d9536e93bda/41598_2023_30259_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/bd482d3d1bcf/41598_2023_30259_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34f3/9950132/9f01b0646721/41598_2023_30259_Fig5_HTML.jpg

相似文献

1
Development of a chub mackerel with less-aggressive fry stage by genome editing of arginine vasotocin receptor V1a2.通过血管加压素受体 V1a2 的基因编辑培育攻击性较弱的幼鲐。
Sci Rep. 2023 Feb 23;13(1):3190. doi: 10.1038/s41598-023-30259-x.
2
An essential role of the arginine vasotocin system in mate-guarding behaviors in triadic relationships of medaka fish (Oryzias latipes).精氨酸血管加压素系统在青鳉鱼（Oryzias latipes）三角关系中的配偶保卫行为中起着重要作用。
PLoS Genet. 2015 Feb 26;11(2):e1005009. doi: 10.1371/journal.pgen.1005009. eCollection 2015.
3
Molecular characterization and expression of arginine vasotocin V1a2 receptor in Atlantic croaker brain: Potential mechanisms of its downregulation by PCB77.大西洋军曹鱼脑中精氨酸加压素 V1a2 受体的分子特征及其表达：PCB77 下调其表达的潜在机制
J Biochem Mol Toxicol. 2020 Jul;34(7):e22500. doi: 10.1002/jbt.22500. Epub 2020 Mar 27.
4
Potential roles of arginine-vasotocin in the regulation of aggressive behavior in the mudskipper (Periophthalmus modestus).精氨酸加压素在弹涂鱼（Periophthalmus modestus）攻击性行为调节中的潜在作用。
Gen Comp Endocrinol. 2013 Dec 1;194:257-63. doi: 10.1016/j.ygcen.2013.09.023. Epub 2013 Oct 7.
5
Arginine vasotocin, isotocin and nonapeptide receptor gene expression link to social status and aggression in sex-dependent patterns.精氨酸血管加压素、异催产素和九肽受体基因表达以性别依赖模式与社会地位和攻击行为相关联。
J Neuroendocrinol. 2015 Feb;27(2):142-57. doi: 10.1111/jne.12239.
6
Variation in gene transcript profiles of two V1a-type arginine vasotocin receptors among sexual phases of bluehead wrasse (Thalassoma bifasciatum).两种蓝头隆头鱼（Thalassoma bifasciatum）性阶段 V1a 型精氨酸加压素受体的基因转录谱变化。
Gen Comp Endocrinol. 2012 Dec 1;179(3):451-64. doi: 10.1016/j.ygcen.2012.10.001. Epub 2012 Oct 12.
7
Molecular characterization, tissue distribution, and mRNA expression profiles of two Kiss genes in the adult male and female chub mackerel (Scomber japonicus) during different gonadal stages.两种 Kiss 基因在雌雄鲐鱼（Scomber japonicus）不同性腺发育阶段的分子特征、组织分布和 mRNA 表达谱。
Gen Comp Endocrinol. 2010 Oct;169(1):28-38. doi: 10.1016/j.ygcen.2010.07.011. Epub 2010 Jul 29.
8
Changes in the expression of pituitary gonadotropin subunits during reproductive cycle of multiple spawning female chub mackerel Scomber japonicus.鲐鱼繁殖周期中脑垂体促性腺激素亚基表达的变化。
Fish Physiol Biochem. 2012 Jun;38(3):883-97. doi: 10.1007/s10695-011-9576-y. Epub 2011 Nov 23.
9
Fatty acids, selenium, and vitamin B12 in chub mackerel (Scomber colias) as nourishment considering seasonality and bioaccessibility as factors.考虑到季节性和生物可利用性因素，竹荚鱼（Scomber colias）中的脂肪酸、硒和维生素 B12 作为营养物质。
Food Chem. 2023 Mar 1;403:134455. doi: 10.1016/j.foodchem.2022.134455. Epub 2022 Sep 29.
10
Differential activation of vasotocin neurons in contexts that elicit aggression and courtship.在引发攻击和求偶行为的情境中，血管紧张素神经元的差异性激活。
Behav Brain Res. 2017 Jan 15;317:188-203. doi: 10.1016/j.bbr.2016.09.008. Epub 2016 Sep 5.

引用本文的文献

1
Modeling the SDF-1/CXCR4 protein using advanced artificial intelligence and antagonist screening for Japanese anchovy.利用先进人工智能对日本鳀鱼的SDF-1/CXCR4蛋白进行建模及拮抗剂筛选。
Front Physiol. 2024 Feb 2;15:1349119. doi: 10.3389/fphys.2024.1349119. eCollection 2024.

本文引用的文献

1
The 2016 red tide crisis in southern Chile: Possible influence of the mass oceanic dumping of dead salmons.智利南部 2016 年赤潮危机：可能是由于大量海洋倾倒死三文鱼所致。
Mar Pollut Bull. 2020 Jan;150:110603. doi: 10.1016/j.marpolbul.2019.110603. Epub 2019 Nov 26.
2
Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis).铂类 TALEN 靶向突变兰尼碱受体导致东方蓝鳍金枪鱼（Thunnus orientalis）游动缓慢。
Sci Rep. 2019 Sep 25;9(1):13871. doi: 10.1038/s41598-019-50418-3.
3
Comprehensive Experimental System for a Promising Model Organism Candidate for Marine Teleosts.
海洋硬骨鱼类有前途的模式生物候选的综合实验系统。
Sci Rep. 2019 Mar 20;9(1):4948. doi: 10.1038/s41598-019-41468-8.
4
Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System.通过 CRISPR/Cas9 系统对受精卵进行基因编辑，生成肌肉生长抑制素基因编辑的斑点叉尾鮰（Ictalurus punctatus）。
Sci Rep. 2017 Aug 4;7(1):7301. doi: 10.1038/s41598-017-07223-7.
5
Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp.利用CRISPR-Cas9对sp7和肌肉生长抑制素进行靶向破坏，导致鲤鱼出现严重的骨骼缺陷和更多的肌肉细胞。
Sci Rep. 2016 Mar 15;6:22953. doi: 10.1038/srep22953.
6
An essential role of the arginine vasotocin system in mate-guarding behaviors in triadic relationships of medaka fish (Oryzias latipes).精氨酸血管加压素系统在青鳉鱼（Oryzias latipes）三角关系中的配偶保卫行为中起着重要作用。
PLoS Genet. 2015 Feb 26;11(2):e1005009. doi: 10.1371/journal.pgen.1005009. eCollection 2015.
7
Targeted mutagenesis using CRISPR/Cas system in medaka.利用 CRISPR/Cas 系统在青鳉中进行靶向诱变。
Biol Open. 2014 Apr 11;3(5):362-71. doi: 10.1242/bio.20148177.
8
Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity.DNA结合模块中非RVD变异的重复模式增强了TALEN活性。
Sci Rep. 2013 Nov 29;3:3379. doi: 10.1038/srep03379.
9
Potential roles of arginine-vasotocin in the regulation of aggressive behavior in the mudskipper (Periophthalmus modestus).精氨酸加压素在弹涂鱼（Periophthalmus modestus）攻击性行为调节中的潜在作用。
Gen Comp Endocrinol. 2013 Dec 1;194:257-63. doi: 10.1016/j.ygcen.2013.09.023. Epub 2013 Oct 7.
10
The fifth neurohypophysial hormone receptor is structurally related to the V2-type receptor but functionally similar to V1-type receptors.第五种神经垂体激素受体在结构上与 V2 型受体相关，但在功能上类似于 V1 型受体。
Gen Comp Endocrinol. 2012 Sep 15;178(3):519-28. doi: 10.1016/j.ygcen.2012.07.008. Epub 2012 Jul 16.