Department of Pharmacology, Faculty of Pharmacy, IFTM University, Moradabad, Uttar Pradesh, 244102, India.
Advanced Phytochemistry Laboratory, Pharmacy Academy, IFTM University, Moradabad, Uttar Pradesh, 244102, India.
Curr Drug Discov Technol. 2023;20(3):e210223213867. doi: 10.2174/1570163820666230221154851.
The root bark of Berberis aristata has been utilized by indigenous peoples for wound treatment for centuries. The mature root barks are crushed into a paste and applied to the wound's surface.
The focus of this research is to analyse the wound healing activities of an ethanolic extract of Berberis aristata, as well as to use molecular docking to establish the likely mechanism of the potent phytochemical. There is no scientific evidence to support the usage of root bark extract of Berberis aristata.
The Herbal ointment, which comprises (1%, 2%, and 4% w/w) ethanolic extract of root bark, was developed to test the wound healing ability of incision and excision wounds, and the molecular mechanism was established using Auto-Dock software.
Epithelization stage, wound index, % wound contraction area, hydroxyproline content, DNA estimate, and histopathological assessments were performed on the incision wound model. Tensile strength was assessed in an excision wound model. TLC was used to identify the samples after successive extractions with different solvents based on polarity.
Berberine and tetrahydropalmatine were major active phytoconstituent found in root barks of Berberis aristata as secondary metabolites. Animals treated with 4% w/w formulation demonstrated considerable wound contraction, epithelization time, and wound index in the excision model. In contrast, to control and standardize the concentrations of hydroxyproline, total amino acids, and DNA in recovering tissue were higher. At 4% w/w extract formulation, the parameters studied indicated a substantial result. Berberine and tetrahydropalmatine, active metabolites which are present in the ethanolic extract of Berberis aristata, were found to be responsible for wound healing. Based on ligand interactions, the findings verified Berberis aristata ethnomedicinal claim in a wound healing capacity.
贯叶连翘的根皮在几个世纪以来一直被原住民用于伤口治疗。将成熟的根皮压碎成糊状物，然后涂抹在伤口表面。
本研究的重点是分析贯叶连翘的乙醇提取物的伤口愈合活性，并使用分子对接来建立潜在的植物化学物质的可能机制。目前没有科学证据支持贯叶连翘根皮提取物的使用。
开发了含有（1%、2%和 4%w/w）根皮乙醇提取物的草药软膏，以测试切口和切除伤口的愈合能力，并使用 Auto-Dock 软件建立分子机制。
在切口伤口模型上进行了上皮化阶段、伤口指数、%伤口收缩面积、羟脯氨酸含量、DNA 估计和组织病理学评估。在切除伤口模型上评估了拉伸强度。根据极性，使用连续提取不同溶剂的 TLC 来鉴定样品。
在贯叶连翘的根皮中发现了小檗碱和延胡索乙素等主要的活性植物成分，它们是次生代谢产物。在切除模型中，用 4%w/w 制剂处理的动物表现出相当大的伤口收缩、上皮化时间和伤口指数。相比之下，控制和标准化恢复组织中的羟脯氨酸、总氨基酸和 DNA 的浓度更高。在 4%w/w 提取物制剂中，研究的参数表明结果显著。存在于贯叶连翘乙醇提取物中的活性代谢物小檗碱和延胡索乙素被认为是伤口愈合的原因。基于配体相互作用，研究结果验证了贯叶连翘在伤口愈合能力方面的民族医学主张。
