Singh Jyotsna, Kakkar Poonam
Herbal Research Section, Indian Institute of Toxicology Research (Council of Scientific & Industrial Research), Post Box No. 80, Mahatma Gandhi Marg, Lucknow 226001, UP, India.
J Ethnopharmacol. 2009 May 4;123(1):22-6. doi: 10.1016/j.jep.2009.02.038. Epub 2009 Mar 5.
Berberis aristata DC root is used in traditional medicine for a number of ailments including metabolic disorders.
The aim of the present study was to explore the antihyperglycemic and antioxidant potential of 50% aqueous ethanolic root extract of Berberis aristata (BA) in alloxan induced diabetic rats.
BA root extract (250 mg/kg) was administered to diabetic rats and standard drug glybenclamide (0.6 mg/kg) to group serving as positive control. Effect of extract on antioxidant and carbohydrate metabolism regulating enzymes of liver was studied in diabetic rats along with its safety parameters.
The main constituents of root were identified as berberine, berbamine and palmatine through HPTLC. The extract besides being safe, lowered the blood glucose significantly without any hypoglycemic effect on their control counterparts. It increased CAT, SOD, GPx, GR activity significantly and reduced lipid peroxidation (41.6%) and protein carbonylation (30.15%). It also increased the glucokinase and glucose-6-phosphate dehydrogenase activities and decreased glucose-6-phosphatase activity in diabetic rats which play a critical role in glucose homeostasis.
Thus, the extract of Berberis aristata (root) has strong potential to regulate glucose homeostasis through decreased gluconeogenesis and oxidative stress.
刺檗根在传统医学中用于治疗多种疾病，包括代谢紊乱。
本研究旨在探讨刺檗50%乙醇水提取物对四氧嘧啶诱导的糖尿病大鼠的降血糖和抗氧化潜力。
将刺檗根提取物（250mg/kg）给予糖尿病大鼠，标准药物格列本脲（0.6mg/kg）给予作为阳性对照的组。研究了提取物对糖尿病大鼠肝脏抗氧化和碳水化合物代谢调节酶的影响及其安全性参数。
通过高效薄层色谱法鉴定出根的主要成分是小檗碱、小檗胺和巴马汀。该提取物除安全外，还显著降低了血糖，对其对照大鼠无任何降血糖作用。它显著提高了过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶的活性，降低了脂质过氧化（41.6%）和蛋白质羰基化（30.15%）。它还增加了糖尿病大鼠的葡萄糖激酶和葡萄糖-6-磷酸脱氢酶的活性，降低了葡萄糖-6-磷酸酶的活性，这些酶在葡萄糖稳态中起关键作用。
因此，刺檗（根）提取物具有通过减少糖异生和氧化应激来调节葡萄糖稳态的强大潜力。
