Digestive Disease Center, CHA Bundang Medical Center, CHA University, Seongnam, Korea.
Yonsei Med J. 2023 Mar;64(3):167-174. doi: 10.3349/ymj.2022.0342.
There are no effective treatment methods with which to control complications of radiation proctitis with fistula or recurrent bleeding following radiation treatment for prostate, cervical, or rectal cancer. Mesenchymal stem cells (MSCs) can induce immune modification, resulting in tissue repair and regeneration. Therefore, we used a rat model of radiation-induced proctitis and observed the effects of using human placenta-derived (PD) and adipose tissue-derived (AD) MSCs.
Female Sprague Dawley rats were irradiated at the pelvic area with 25 Gy. We injected 1×10 cells of human PD-MSCs, human AD-MSCs, human foreskin fibroblasts, and control media into the rectal submucosa following irradiation. We sacrificed rats for pathologic evaluation.
Fibrosis on the rectum was reduced in both MSC groups, compared to the control group. Mucosal Ki-67 indices of both MSC injected groups were higher than those in the control group. Although caspase-3 positive cells in the mucosa gradually increased and decreased in the control group, those in both MSC injected groups increased rapidly and decreased thereafter.
We demonstrated the effects of regional MSC injection treatment for radiation-induced proctitis in rats. MSC injection reduced fibrosis and increased proliferation in rat mucosa. Human AD-MSCs and PD-MSCs had similar effectiveness.
对于前列腺癌、宫颈癌或直肠癌放射治疗后并发瘘管或反复出血的放射性直肠炎，目前尚无有效的治疗方法。间充质干细胞（MSCs）可以诱导免疫调节，从而促进组织修复和再生。因此，我们使用大鼠放射性直肠炎模型，观察了人胎盘来源（PD）和脂肪组织来源（AD）MSCs 的作用。
雌性 Sprague Dawley 大鼠在盆腔区域接受 25Gy 照射。照射后，我们将 1×10 个细胞的人 PD-MSCs、人 AD-MSCs、人包皮成纤维细胞和对照培养基注入直肠黏膜下层。我们对大鼠进行病理评估。
与对照组相比，两组 MSC 组的直肠纤维化均减少。MSC 注射组的黏膜 Ki-67 指数均高于对照组。虽然对照组的黏膜中 caspase-3 阳性细胞逐渐增加并减少，但 MSC 注射组的细胞迅速增加并减少。
我们证明了局部 MSC 注射治疗大鼠放射性直肠炎的效果。MSC 注射减少了大鼠黏膜的纤维化并增加了增殖。人 AD-MSCs 和 PD-MSCs 具有相似的效果。
