Department of Inorganic and Analytical Chemistry, NMR Group, Budapest University of Technology and Economics, H-1111 Budapest, Hungary.
Institute of Biophysics, Biological Research Centre, Szeged H-6726, Hungary.
J Nat Prod. 2023 Apr 28;86(4):1074-1080. doi: 10.1021/acs.jnatprod.2c00948. Epub 2023 Feb 24.
Ecdysteroid-containing herbal extracts, commonly prepared from the roots of , are marketed worldwide as a "green" anabolic food supplement. Herein are reported the isolation and complete H and C NMR signal assignments of three new minor ecdysteroids (compounds -) from this extract. Compound was identified as a possible artifact that gradually forms through the autoxidation of calonysterone. The compounds tested demonstrated a significant protective effect on the blood-brain barrier endothelial cells against oxidative stress or inflammation at a concentration of 1 μM. Based on these results, minor ecdysteroids present in food supplements may offer health benefits in various neurodegenerative disease states.
含蜕皮甾酮的草药提取物，通常从 的根部提取，作为一种“绿色”的合成代谢食品补充剂在全球范围内销售。本文报道了从这种提取物中分离出的三种新的微量蜕皮甾酮（化合物 -）的结构鉴定和 1 H 和 13 C NMR 全信号归属。化合物 被鉴定为可能的人工产物，它通过 calonysterone 的自动氧化逐渐形成。在 1 μM 的浓度下，这些化合物对血脑屏障内皮细胞的氧化应激或炎症具有显著的保护作用。基于这些结果，食品补充剂中存在的微量蜕皮甾酮可能在各种神经退行性疾病状态下提供健康益处。
