高效离子交换色谱-串联质谱法测定早产儿中咖啡因、可可碱、茶碱和可可因
Determination of caffeine, paraxanthine, theophylline and theobromine in premature infants by HILIC-MS/MS.
机构信息
Research Division of Clinical Pharmacology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.
School of Basic Medicine & Clinical Pharmacy, China Pharmaceutical University, Nanjing, 210009, China.
出版信息
Bioanalysis. 2022 Dec;14(23):1497-1508. doi: 10.4155/bio-2022-0131. Epub 2023 Feb 24.
Caffeine is a central nervous system stimulant, used to treat apnea of prematurity. A hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) approach was developed to detect caffeine, paraxanthine, theophylline and theobromine in premature infants. Protein precipitation of plasma samples (10 μl) was carried out by treating with acetonitrile containing caffeine-C. The separation was carried out on an ACQUITY HPLC BEH HILIC column. Caffeine and its metabolites were quantified by multiple reaction monitoring modes with positive electrospray ionization. The established method had a good linear relationship in the range of 0.0600-60.0 μg/ml for caffeine, 0.0250-7.50 μg/ml for theobromine and 0.0150-4.50 μg/ml for paraxanthine and theophylline. A HILIC-MS/MS method was developed and validated to determine caffeine and its major metabolites in plasma of premature infants.
咖啡因是一种中枢神经系统兴奋剂，用于治疗早产儿呼吸暂停。建立了一种亲水作用色谱-串联质谱（HILIC-MS/MS）方法，用于检测早产儿血浆中的咖啡因、可可碱、茶碱和可可因。通过用含有咖啡因-C 的乙腈处理来沉淀血浆样品（10 μl）中的蛋白质。在 ACQUITY HPLC BEH HILIC 柱上进行分离。采用正电喷雾电离多反应监测模式定量检测咖啡因及其代谢物。该方法在咖啡因的浓度范围为 0.0600-60.0 μg/ml、可可因的浓度范围为 0.0250-7.50 μg/ml、可可因和茶碱的浓度范围为 0.0150-4.50 μg/ml 时具有良好的线性关系。建立并验证了一种亲水作用色谱-串联质谱法，用于检测早产儿血浆中的咖啡因及其主要代谢物。
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