Pessanha Fernanda Soares, Oliveira Beatriz Guitton Renaud Baptista de, Oliveira Bianca Campos, Deutsch Gabriela, Teixeira Felipe Lopes, Bokehi Luciana Castilho, Calomino Mariana Alcântara, Rodrigues de Castilho Selma, Thiré Rossana Mara da Silva Moreira, Teixeira Lenise Arneiro, Paula Geraldo Renato de
Nursing School, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20551-030, Brazil.
Aurora de Afonso Costa College of Nursing, Universidade Federal Fluminense, Niterói 24020-091, Brazil.
Gels. 2023 Feb 1;9(2):117. doi: 10.3390/gels9020117.
Diabetic patients frequently develop wounds, which can be colonized by bacteria, mainly and , with the ability to form biofilms. This study aimed to evaluate the colonization and biofilm formation of and in chronic wounds of diabetic patients treated with a bioactive dressing (EGF-CMC), which consisted of a 2% carboxymethylcellulose (CMC) hydrogel loaded with epidermal growth factor (EGF). This randomized clinical trial was conducted with 25 participants: 14 treated with EGF-CMC hydrogel and 11 treated with CMC hydrogel for 12 weeks. Participants with type 2 diabetes mellitus were selected. All had diabetic foot ulcers or chronic venous ulcers. Swab collections were performed on weeks 1, 6, and 12. The laboratory analyses included the identification of strains, microbial quantification, virulence gene investigation, and the evaluation of biofilm formation. In total, 13 strains and 15 strains were isolated. There were no statistically significant differences regarding bacterial loads and virulence genes. However, EGF-CMC-hydrogel-treated wounds were colonized by strains with lower biofilm formation abilities. The probability of isolating biofilm-producing strains from CMC-hydrogel-treated wounds was 83% greater than the probability of isolating biofilm-producing strains from EGF-CMC-treated wounds.
糖尿病患者经常出现伤口，这些伤口可能被细菌定植，主要是[具体细菌1]和[具体细菌2]，它们具有形成生物膜的能力。本研究旨在评估[具体细菌1]和[具体细菌2]在接受生物活性敷料（EGF-CMC）治疗的糖尿病患者慢性伤口中的定植和生物膜形成情况，该敷料由负载表皮生长因子（EGF）的2%羧甲基纤维素（CMC）水凝胶组成。这项随机临床试验纳入了25名参与者：14名接受EGF-CMC水凝胶治疗，11名接受CMC水凝胶治疗，为期12周。入选的是2型糖尿病患者。所有患者均患有糖尿病足溃疡或慢性静脉溃疡。在第1、6和12周进行拭子采集。实验室分析包括菌株鉴定、微生物定量、毒力基因检测以及生物膜形成评估。总共分离出13株[具体细菌1]菌株和15株[具体细菌2]菌株。在细菌载量和毒力基因方面没有统计学上的显著差异。然而，接受EGF-CMC水凝胶治疗的伤口被生物膜形成能力较低的菌株定植。从接受CMC水凝胶治疗的伤口中分离出产生生物膜菌株的概率比从接受EGF-CMC治疗的伤口中分离出产生生物膜菌株的概率高83%。
