Zheng Xiao, Liu Yu, Liu Yangxi, Pan Yining, Yao Qingqing
School of Ophthalmology and Optometry, Wenzhou Medical University, Wenzhou, Zhejiang, China.
Institute of Advanced Materials for Nano-Bio Applications, Wenzhou Medical University, Wenzhou, Zhejiang, China.
J Biomed Mater Res B Appl Biomater. 2021 Apr;109(4):517-526. doi: 10.1002/jbm.b.34720. Epub 2020 Aug 31.
The clinical use of FDA-approved bone morphogenetic proteins (BMPs) are impeded by high costs, super-high dosage requirement, short half-life, and other undesirable side effects. Therefore, designing a biomaterial that can promote new bone formation without using exogenous BMPs is highly desirable in clinical applications. In the present work, a new kind of nanofibrous scaffold composed of gelatin and 45S5 bioglass (GF/45S5 BG) was prepared through thermally induced phase separation method together with the particle leach technique (TIPS&P). In addition to the significantly higher mechanical strength, the composite scaffolds (GF/45S5 BG) significantly increased osteogenic differentiation of human mesenchymal stem cells (hMSCs) in vitro compared with the neat scaffold (GF) without adding other biological agents, for example, BMPs or hormones. Most importantly, our in vivo studies also indicated that GF/45S5 BG scaffolds could directly promote ectopic bone regeneration in SD rats without exogenous BMP2. In summary, both in vitro and in vivo results indicated that the novel 45S5 bioglass functionalized GF nanofibrous scaffold is a promising alternative for bone tissue engineering.
美国食品药品监督管理局（FDA）批准的骨形态发生蛋白（BMPs）的临床应用受到高成本、超高剂量需求、半衰期短以及其他不良副作用的阻碍。因此，设计一种无需使用外源性BMPs就能促进新骨形成的生物材料在临床应用中非常必要。在本研究中，通过热诱导相分离法结合粒子沥滤技术（TIPS&P）制备了一种由明胶和45S5生物玻璃组成的新型纳米纤维支架（GF/45S5 BG）。除了具有显著更高的机械强度外，与未添加其他生物制剂（如BMPs或激素）的纯支架（GF）相比，复合支架（GF/45S5 BG）在体外显著增加了人间充质干细胞（hMSCs）的成骨分化。最重要的是，我们的体内研究还表明，GF/45S5 BG支架无需外源性BMP2就能直接促进SD大鼠的异位骨再生。总之，体外和体内结果均表明，新型45S5生物玻璃功能化的GF纳米纤维支架是骨组织工程的一种有前景的替代材料。
