Department Functional Interfaces, Leibniz-Institute of Photonic Technology Jena, Albert-Einstein-Straße 9, 07745, Jena, Germany.
Institute of Physical Chemistry, Friedrich-Schiller University Jena, Helmholtzweg 4, 07743, Jena, Germany.
Angew Chem Int Ed Engl. 2023 Apr 17;62(17):e202301452. doi: 10.1002/anie.202301452. Epub 2023 Mar 20.
This study employs TLD1433, a Ru -based photodynamic therapy (PDT) agent in human clinical trials, as a benchmark to establish protocols for studying the excited-state dynamics of photosensitizers (PSs) in cellulo, in the local environment provided by human cancer cells. Very little is known about the excited-state properties of any PS in live cells, and for TLD1433, it is terra incognita. This contribution targets a general problem in phototherapy, which is how to interrogate the light-triggered, function-determining processes of the PSs in the relevant biological environment, and establishes methodological advances to study the ultrafast photoinduced processes for TLD1433 when taken up by MCF7 cells. We generalize the methodological developments and results in terms of molecular physics by applying them to TLD1433's analogue TLD1633, making this study a benchmark to investigate the excited-state dynamics of phototoxic compounds in the complex biological environment.
本研究采用 TLD1433，一种正在进行人体临床试验的钌基光动力治疗（PDT）试剂，作为基准来建立在人类癌细胞提供的局部环境中研究细胞内光敏剂（PS）激发态动力学的方案。对于任何在活细胞中的 PS 的激发态性质，人们知之甚少，而对于 TLD1433，更是知之甚少。本研究针对光疗中的一个普遍问题，即如何在相关的生物环境中探究 PS 的光触发、功能决定过程，并为 MCF7 细胞摄取 TLD1433 时研究其超快光诱导过程建立了方法学进展。我们通过将这些方法和结果应用于 TLD1433 的类似物 TLD1633，从分子物理的角度对其进行了概括，使本研究成为一个基准，以研究复杂生物环境中光毒性化合物的激发态动力学。
