Tucker A N, Tang T, Friedman M A
J Environ Pathol Toxicol. 1978 Nov-Dec;2(2):571-8.
Administration of N-nitrosodiethylamine (diethylnitrosamine, DEN) to mice caused a loss of cytochrome P-450 and a corresponding depression in the activities of aminopyrine demethylase and aniline hydroxylase. Maximum effects were achieved 24 hr. after a single dose of 100 mg/kg. In chronic experiments, similar effects were achieved after animals had been drinking water containing 50 ppm of DEN for 12 weeks. The effects of DEN on aminopyrine demethylase could not be reproduced by collecting microsomes, from homogenates which had been treated with DEN in vitro. Homogenates prepared from livers of mice treated chronically with DEN were used to activate compounds to mutagens in the Salmonella/microsome test of Ames. Activation by these homogenates was not lower than activation by homogenates prepared from control animals. In fact, activation of aflatoxin B1 was enhanced by use of homogenates from DEN-treated animals as source of activating enzymes.
给小鼠施用N-亚硝基二乙胺（二乙基亚硝胺，DEN）会导致细胞色素P-450的丧失，以及氨基比林脱甲基酶和苯胺羟化酶活性相应降低。单次剂量100mg/kg后24小时达到最大效应。在慢性实验中，动物饮用含50ppm DEN的水12周后也出现了类似效应。通过收集体外经DEN处理的匀浆中的微粒体，无法重现DEN对氨基比林脱甲基酶的影响。在Ames沙门氏菌/微粒体试验中，用长期经DEN处理的小鼠肝脏制备的匀浆来激活化合物使其成为诱变剂。这些匀浆的激活作用不低于用对照动物制备的匀浆的激活作用。事实上，使用经DEN处理动物的匀浆作为激活酶来源时，黄曲霉毒素B1的激活作用增强。
