Siraj M Y, Hayes A W, Takanaka A, Ho I K
J Environ Pathol Toxicol. 1980 Sep;4(2-3):545-53.
The in vivo effects of patulin on hepatic monooxygenase enzymes in male mice were examined after single and multiple exposures (i.p.) to the mycotoxin. The animals were exposed to a single dose of 1, 3 or 4.5 mg patulin/kg or daily doses of 1.0 mg/kg for 5 or 14 days. After single exposure no significant effect was observed on hexobarbital hydroxylation while aniline hydroxylase increased significantly at 3 mg/kg and at 48 hr. The demethylation of aminopyrine and ethylmorphine increased significantly 48 and 72 hr after 3.0 or 4.5 mg patulin/kg doses. A 12% increase was observed in cytochrome P-450 content 48 hr after exposure to 1 or 3 mg/kg. NADPH-cytochrome C reductase was enhanced significantly at all 3 dose levels at 48 hr. At 24 hr hepatic NADPH-dependent dehydrogenase activity increased 38 and 46% in animals exposed to a single dose of 3 mg patulin/kg, respectively. No effect of patulin was observed on the hepatic drug metabolizing enzymes examined except hexobarbital oxidation in mice exposed to multiple doses of patulin. Patulin at best was a weak inducer of the hepatic mixed function oxidase system.
在雄性小鼠经腹腔注射单次或多次暴露于展青霉素后，检测了展青霉素对其肝脏单加氧酶的体内效应。动物分别单次暴露于1、3或4.5毫克展青霉素/千克剂量，或每日暴露于1.0毫克/千克剂量，持续5天或14天。单次暴露后，未观察到对己巴比妥羟化有显著影响，而在3毫克/千克剂量且48小时时苯胺羟化酶显著增加。在3.0或4.5毫克展青霉素/千克剂量后48小时和72小时，氨基比林和乙基吗啡的去甲基化显著增加。暴露于1或3毫克/千克剂量48小时后，细胞色素P - 450含量增加了12%。在48小时时，所有3个剂量水平的NADPH - 细胞色素C还原酶均显著增强。在24小时时，单次暴露于3毫克展青霉素/千克剂量的动物肝脏NADPH依赖性脱氢酶活性分别增加了38%和46%。除了多次暴露于展青霉素的小鼠中的己巴比妥氧化外，未观察到展青霉素对所检测的肝脏药物代谢酶有影响。展青霉素充其量只是肝脏混合功能氧化酶系统的弱诱导剂。
