Mohd-Azami Siti Nurul Izzah, Loong Shih Keng, Khoo Jing Jing, Husin Nurul Aini, Lim Fang Shiang, Mahfodz Nur Hidayana, Ishak Siti Nabilah, Mohd-Taib Farah Shafawati, Makepeace Benjamin L, AbuBakar Sazaly
Tropical Infectious Diseases Research & Education Centre (TIDREC), Higher Institution Centre of Excellence, Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Institute for Advanced Studies (IAS), Universiti Malaya, Kuala Lumpur 50603, Malaysia.
Trop Med Infect Dis. 2023 Jan 19;8(2):74. doi: 10.3390/tropicalmed8020074.
Many human clinical cases attributed to vector-borne pathogens are underreported in Malaysia, especially in rural localities where healthcare infrastructures are lacking. Here, 217 small mammals, consisting of rodents and tree shrews, were trapped in oil palm plantations in the Peninsular Malaysia states of Johor and Perak. Species identification was performed using morphological and DNA barcoding analyses, and 203 small mammals were included in the detection of selected vector-borne bacteria. The DNA extracted from the spleens was examined for , spp., spp. and spp. using established PCR assays. The small mammals collected in this study included R3 mitotype ( = 113), ( = 24), ( = 22), ( = 17), ( = 1) and ( = 40). , spp. and were detected in the small mammals with the respective detection rates of 12.3%, 5.9% and 4.9%. spp., however, was not detected. This study encountered the presence of both Lyme disease and relapsing fever-related borreliae in small mammals collected from the oil palm plantation study sites. All three microorganisms (, spp. and ) were detected in the R3 mitotype, suggesting that the species is a competent host for multiple microorganisms. Further investigations are warranted to elucidate the relationships between the ectoparasites, the small mammals and the respective pathogens.
在马来西亚，许多由媒介传播病原体引起的人类临床病例报告不足，尤其是在缺乏医疗基础设施的农村地区。在此，217只小型哺乳动物，包括啮齿动物和树鼩，在马来西亚半岛柔佛州和霹雳州的油棕种植园中被捕获。使用形态学和DNA条形码分析进行物种鉴定，203只小型哺乳动物被纳入选定的媒介传播细菌的检测。使用既定的PCR检测方法，检查从脾脏中提取的DNA是否存在 、 属、 属和 属。本研究收集的小型哺乳动物包括R3基因型（ = 113）、 （ = 24）、 （ = 22）、 （ = 17）、 （ = 1）和 （ = 40）。在小型哺乳动物中检测到 、 属和 ，其各自的检测率分别为12.3%、5.9%和4.9%。然而，未检测到 属。本研究在从油棕种植园研究地点收集的小型哺乳动物中发现了莱姆病和回归热相关的疏螺旋体。在R3基因型中检测到了所有三种微生物（ 、 属和 ），这表明该物种是多种微生物的适宜宿主。有必要进行进一步调查，以阐明体外寄生虫、小型哺乳动物和各自病原体之间的关系。
