Oberprieler Christoph, Ott Tankred, Vogt Robert
Evolutionary and Systematic Botany Group, Institute of Plant Biology, University of Regensburg, Universitätsstr. 31, D-93053 Regensburg, Germany.
Botanic Garden & Botanical Museum Berlin, Freie Universität Berlin, Königin-Luise-Str. 6-8, D-14191 Berlin, Germany.
Biology (Basel). 2023 Feb 10;12(2):288. doi: 10.3390/biology12020288.
Based on the results of a preceding species-delimitation analysis for the diploid representatives of the genus (Compositae, Anthemideae), the present study aims at the elaboration of a specific and subspecific taxonomic treatment of the tetraploid members of the genus. Following an integrative taxonomic approach, species-level decisions on eight predefined morphotaxon hypotheses were based on genetic/genealogical, morphological, ecological, and geographical differentiation patterns. ddRADseq fingerprinting and SNP-based clustering revealed genetic integrity for six of the eight morphotaxa, with no clear differentiation patterns observed between the widespread subsp. and the N Spanish (Cordillera Cantábrica) and the S French subsp. (northern Massif Central) and (western Massif Central). The inclusion of differentiation patterns in morphological (leaf dissection and shape), ecological (climatological and edaphic niches), and geographical respects (pair-wise tests of sympatry vs. allopatry) together with the application of a procedural protocol for species-rank decisions (the 'Wettstein tesseract') led to the proposal of an acknowledgement of the eight predefined morphotaxon hypotheses as six species (two of them with two subspecies). Nomenclatural consequences following from these results are drawn and lead to the following new combinations: subsp. (Legrand) Oberpr., T.Ott & Vogt, comb. nov. and (Jeanb. & Timb.-Lagr.) Oberpr., T.Ott & Vogt, comb. et stat. nov.
基于之前对该属（菊科，春黄菊族）二倍体代表物种进行的物种界定分析结果，本研究旨在对该属四倍体成员进行特定和亚种层面的分类处理。遵循综合分类学方法，基于遗传/谱系、形态、生态和地理分化模式，对八个预先定义的形态分类假设做出物种层面的判定。ddRADseq指纹图谱和基于单核苷酸多态性的聚类分析表明，八个形态分类单元中有六个具有遗传完整性，在广泛分布的[具体亚种名1]亚种与西班牙北部（坎塔布连山脉）的[具体亚种名2]以及法国南部（中央高原北部）的[具体亚种名3]和（中央高原西部）之间未观察到明显的分化模式。将形态（叶裂和形状）、生态（气候和土壤生态位）和地理方面的分化模式（同域分布与异域分布的成对检验）纳入考量，并应用物种等级判定的程序协议（“韦特施泰因立方体”），从而提议将八个预先定义的形态分类假设确认为六个物种（其中两个物种各有两个亚种）。得出这些结果后的命名学后果，并导致以下新组合：[具体组合1]亚种（勒格朗）奥伯普、T. 奥特和沃格特，新组合。以及[具体组合2]（让布 & 廷布尔 - 拉格朗）奥伯普、T. 奥特和沃格特，新组合及新状态。
