Scoparo Melissa, Cardinali Irene, La Porta Gianandrea, Caldaroni Barbara, Magara Gabriele, Dörr Ambrosius Josef Martin, Elia Antonia Concetta, Lancioni Hovirag
Department of Chemistry, Biology and Biotechnology, Università degli Studi di Perugia, 06123 Perugia, Italy.
Biology (Basel). 2023 Feb 15;12(2):313. doi: 10.3390/biology12020313.
The red swamp crayfish is one of the most threatening freshwater species in the world. The aim of this study is to provide a better understanding of the phylogeography and the invasion routes of populations in the Italian Peninsula through the analysis of mitochondrial phylogeny. Mitochondrial control region and cytochrome c oxidase subunit I (COI) sequences of 153 samples collected from six Italian basins were analyzed and compared to worldwide data. Except for the lakes Bolsena and Posta Fibreno, a high genetic variability was found in the other basins. The mitochondrial DNA pattern of from the lakes Candia and Massaciuccoli confirmed the hypothesis of double introduction events. Another entry point could be represented by Lake Trasimeno, which shows haplotypes originating from Louisiana and not shared with other Italian basins. Moreover, unique lineages were also found in the Stella River, thus enhancing the hypothesis that multiple introductions of occurred in northern and Central Italy and strengthening the idea that knowledge about the dispersion routes of this alien species can be useful to predict its invasiveness and elaborate control strategies to preserve biodiversity.
红沼泽螯虾是世界上最具威胁性的淡水物种之一。本研究的目的是通过分析线粒体系统发育，更好地了解意大利半岛种群的系统地理学和入侵路线。对从意大利六个流域采集的153个样本的线粒体控制区和细胞色素c氧化酶亚基I（COI）序列进行了分析，并与全球数据进行了比较。除了博尔塞纳湖和波斯塔菲布雷诺湖，在其他流域发现了高度的遗传变异性。坎迪亚湖和马萨基乌科利湖的线粒体DNA模式证实了双重引入事件的假设。另一个引入点可能是特拉西梅诺湖，该湖显示出源自路易斯安那州的单倍型，且其他意大利流域没有共享。此外，在斯特拉河也发现了独特的谱系，从而强化了在意大利北部和中部发生多次引入的假设，并强化了这样一种观点，即了解这种外来物种的扩散路线有助于预测其入侵性，并制定保护生物多样性的控制策略。
