Modesti Margherita, Marchica Alessandra, Pisuttu Claudia, Risoli Samuele, Pellegrini Elisa, Bellincontro Andrea, Mencarelli Fabio, Tonutti Pietro, Nali Cristina
Department for Innovation in Biological, Agro-Food and Forest Systems, University of Tuscia, Via S. Camillo de Lellis, 01100 Viterbo, Italy.
Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
Antioxidants (Basel). 2023 Jan 31;12(2):343. doi: 10.3390/antiox12020343.
To investigate how plants cope with multi-stress conditions, we analyzed the biochemical and molecular changes of leaves subjected to single or sequential double stresses (infection by () and ozone (O, 100 ppb for 3 h) treatment). In Bc/O leaves, the hydrogen peroxide (HO) induction (observed at 12 and 24 h from the end of treatment (FET)) triggered a production of ethylene (Et; +35% compared with Bc/O leaves), which was preceded by an increase of salicylic acid (SA; +45%). This result confirms a crosstalk between SA- and Et-related signaling pathways in lesion spread. The ozone induced an early synthesis of Et followed by jasmonic acid (JA) and SA production (about 2-fold higher), where Et and SA signaling triggered reactive oxygen species production by establishing a feedback loop, and JA attenuated this cycle by reducing Et biosynthesis. In + O leaves, Et peaked at 6 and 12 h FET, before SA confirmed a crosstalk between Et- and SA-related signaling pathways in lesion propagation. In O + leaves, the HO induction triggered an accumulation of JA and Et, demonstrating a synergistic action in the regulation of defence reactions. The divergence in these profiles suggests a rather complex network of events in the transcriptional regulation of genes involved in the systemic acquired resistance.
为了研究植物如何应对多重胁迫条件，我们分析了遭受单一或连续双重胁迫（接种（）和臭氧（O，100 ppb处理3小时））的叶片的生化和分子变化。在Bc/O叶片中，过氧化氢（HO）诱导（在处理结束后12小时和24小时观察到）引发了乙烯（Et）的产生（与Bc/O叶片相比增加了35%），这之前水杨酸（SA）增加（增加了45%）。这一结果证实了SA和Et相关信号通路在病斑扩展中的相互作用。臭氧诱导了Et的早期合成，随后是茉莉酸（JA）和SA的产生（约高2倍），其中Et和SA信号通过建立反馈环触发活性氧的产生，而JA通过减少Et生物合成减弱了这个循环。在+O叶片中，Et在处理结束后6小时和12小时达到峰值，之后SA证实了Et和SA相关信号通路在病斑传播中的相互作用。在O+叶片中，HO诱导引发了JA和Et的积累，表明在防御反应调节中存在协同作用。这些图谱的差异表明，参与系统获得性抗性的基因转录调控中存在相当复杂的事件网络。
