Tvrdá Eva, Petrovičová Michaela, Benko Filip, Ďuračka Michal, Kováč Ján, Slanina Tomáš, Galovičová Lucia, Žiarovská Jana, Kačániová Miroslava
Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia.
Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia.
Antibiotics (Basel). 2023 Feb 5;12(2):336. doi: 10.3390/antibiotics12020336.
This study aimed to characterize the bacterial profiles and their association with selected semen quality traits among two chicken breeds. Thirty Lohmann Brown and thirty ROSS 308 roosters were selected for semen quality estimation, including sperm motility, membrane and acrosome integrity, mitochondrial activity, and DNA fragmentation. The oxidative profile of the semen, including the production of reactive oxygen species (ROS), antioxidant capacity, protein, and lipid oxidation, were assessed as well. Moreover, the levels of pro-inflammatory cytokines, including tumor necrosis factor alpha (TNF-α), interleukins 1 and 6 (IL-1, IL-6) and C-reactive protein, as well as the concentrations of selected antibacterial proteins (cathelicidin, β-defensin and lysozyme) in the seminal plasma were evaluated with the enzyme-linked immunosorbent assay. The prevailing bacterial genera identified by the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry were spp., spp., spp. and spp. While the bacterial load was significantly higher in the ROSS 308 line ( < 0.05), a higher number of potentially uropathogenic bacteria was found in the Lohmann Brown roosters. Antimicrobial susceptibility tests revealed a substantial resistance of randomly selected bacterial strains, particularly to ampicillin, tetracycline, chloramphenicol, and tobramycin. Furthermore, Lohmann Brown ejaculates containing an increased proportion of presented with significantly ( < 0.05) elevated levels of TNF-α and IL-6, as well as ROS overproduction and lipid peroxidation. Inversely, significantly ( < 0.05) higher levels of β-defensin and lysozyme were found in the semen collected from the ROSS 308 roosters, which was characterized by a higher quality in comparison to the Lohmann Brown roosters. In conclusion, we emphasize the criticality of bacteriospermia in the poultry industry and highlight the need to include a more complex microbiological screening of semen samples designated for artificial insemination.
本研究旨在描述两个鸡品种的细菌谱及其与选定精液质量性状的关联。选择了30只罗曼褐公鸡和30只罗斯308公鸡进行精液质量评估，包括精子活力、膜和顶体完整性、线粒体活性以及DNA片段化。还评估了精液的氧化谱，包括活性氧（ROS）的产生、抗氧化能力、蛋白质和脂质氧化。此外，采用酶联免疫吸附测定法评估了促炎细胞因子水平，包括肿瘤坏死因子α（TNF-α）、白细胞介素1和6（IL-1、IL-6）以及C反应蛋白，以及精浆中选定抗菌蛋白（cathelicidin、β-防御素和溶菌酶）的浓度。通过基质辅助激光解吸/电离飞行时间质谱法鉴定出的主要细菌属为 spp.、 spp.、 spp.和 spp.。虽然罗斯308品系的细菌载量显著更高（ < 0.05），但在罗曼褐公鸡中发现了更多潜在的尿路致病性细菌。抗菌药敏试验显示，随机选择的细菌菌株具有相当大的耐药性，尤其是对氨苄青霉素、四环素、氯霉素和妥布霉素。此外，含有比例增加的 的罗曼褐公鸡射精中，TNF-α和IL-6水平显著升高（ < 0.05），同时ROS产生过多和脂质过氧化。相反，在从罗斯308公鸡采集的精液中发现β-防御素和溶菌酶水平显著更高（ < 0.05），与罗曼褐公鸡相比，其精液质量更高。总之，我们强调了家禽业中菌精症的关键性，并强调需要对用于人工授精的精液样本进行更复杂的微生物筛查。
