Department of Psychiatry, Semel Institute for Neuroscience and Human Behavior, The University of California Los Angeles, Los Angeles, CA 90095, USA.
Biomolecules. 2023 Jan 19;13(2):201. doi: 10.3390/biom13020201.
Intracranial hypertension (ICP) and visual impairment intracranial pressure (VIIP) are some of the consequences of long-term space missions. Here we examined the behavior of oligodendrocyte progenitors (OLPs) after space flight using time-lapse microscopy. We show that most OLPs divided more than ground control (GC) counterparts did. Nonetheless, a subpopulation of OLPs flown to space presented a significant increase in autophagic cell death. Examination of the proteomic profile of the secretome of space flown OLPs (SPC-OLPs) revealed that the stress protein heat shock protein-90 beta "HSP-90β" was the 5 most enriched (6.8 times) and the secreted protein acidic and rich in cysteine "SPARC" was the 7 most enriched (5.2 times), with respect to ground control cells. SPARC induces endoplasmic reticulum stress, which leads to autophagy. Given the roles and importance of these two proteins in mammalian cells' metabolism, their upregulation may hold the key to modulating cell proliferation and autophagy, in order to mitigate ICP and VIIP during and after space missions.
颅内高压（ICP）和视觉损害颅内压（VIIP）是长期太空任务的一些后果。在这里，我们使用延时显微镜检查了太空飞行后少突胶质前体细胞（OLP）的行为。我们发现，大多数 OLP 的分裂次数超过了地面对照组。尽管如此，一部分飞往太空的 OLP 出现了明显的自噬细胞死亡增加。对太空飞行 OLP 的分泌组（SPC-OLP）的蛋白质组谱进行检查显示，应激蛋白热休克蛋白 90β“HSP-90β”是最富集的第 5 位（6.8 倍），富含半胱氨酸的酸性分泌蛋白“SPARC”是最富集的第 7 位（5.2 倍），与地面对照组细胞相比。SPARC 诱导内质网应激，导致自噬。鉴于这两种蛋白质在哺乳动物细胞代谢中的作用和重要性，它们的上调可能是调节细胞增殖和自噬的关键，以减轻太空任务期间和之后的 ICP 和 VIIP。
