Renault G, Muffat-Joly M, Polianski J, Hardy R I, Boutineau J L, Duvent J L, Pocidalo J J
INSERM Unité 13, Hôpital Claude Bernard, Paris, France.
Lasers Surg Med. 1987;7(4):339-46. doi: 10.1002/lsm.1900070408.
NADH laser fluorimetry was applied to in situ monitoring of pentobarbital overload effect on myocardial redox state in rats. Experiments were carried out on open-chest rats anesthetized with pentobarbital. Use of very thin optical probes allowed measurements of NADH fluorescence directly within the myocardial wall. Quantitative analysis of fluorescent and backscattered light signals was made possible on-line. In 8 animals, a slow IV infusion of 6% pentobarbital (2 mg.min-1 for 10 min) induced an average twofold increase in NADH fluorescence, with constant return to baseline level prior to injection after a maximum period of 50 min. Thus, pentobarbital overload strongly affects the myocardial energetic state, and this effect was precisely evaluated with NADH fluorescence monitoring. In situ-monitored myocardial redox state variations during pentobarbital infusion can constitute a reference for similar studies on pharmacological agents aimed at protecting tissues against ischemic biochemical damages.
应用NADH激光荧光法对大鼠戊巴比妥过载对心肌氧化还原状态的影响进行原位监测。实验在戊巴比妥麻醉的开胸大鼠身上进行。使用非常细的光学探头可直接测量心肌壁内的NADH荧光。荧光和背散射光信号的定量分析可在线进行。在8只动物中，缓慢静脉输注6%戊巴比妥（2毫克·分钟-1，持续10分钟）使NADH荧光平均增加两倍，在最长50分钟后达到最大值，然后在注射前持续恢复到基线水平。因此，戊巴比妥过载强烈影响心肌能量状态，并且通过NADH荧光监测精确评估了这种影响。戊巴比妥输注期间原位监测的心肌氧化还原状态变化可为旨在保护组织免受缺血性生化损伤的药物类似研究提供参考。
