College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
Hunan Provincial Engineering Technology Research Center for Cell Mechanics and Function Analysis, Changsha 410128, China.
Biosensors (Basel). 2023 Jan 28;13(2):198. doi: 10.3390/bios13020198.
The main sensing techniques used to study myocardial pulsation are electrical impedance sensing (EIS) and by quartz crystal microbalance (QCM). While electrical impedance technology is the gold standard for the study of myocardial pulsation, the clinical application of drugs is being followed up in real time additionally, thus, QCM technology needs to be further developed as a very important class of quality sensor technology. Moreover, the application of EIS, in combination with the QCM, for monitoring myocardial pulsation, has been rarely reported. In this paper, a series of cell growth and adhesion conditions were optimized using rat primary cardiomyocytes, and QCM was used in combination with EIS to monitor the adhesion and the myocardial pulsation ability of the cells in real time. Furthermore, cardiomyocytes that adhered to the QCM and EIS were treated with isoprenaline (ISO), a positive inotropic drug, and verapamil (VRP), a negative inotropic drug. Next, the cell index (CI)-time (T) plots, beating amplitude (BA) and beating rate (BR) of the cardiomyocytes were calculated and changes in these parameters, before and after, dosing were evaluated. The results showed that the QCM technique results were not only consistent with the results obtained with EIS, but also that the QCM technique had a certain degree of sensitivity for the calculation of cardiomyocyte beating. Thus, our findings validate the reliability and validity of the QCM technique for measuring cardiomyocyte beating and drug testing. We hope that further studies would evaluate the application of the QCM technology for clinical use.
用于研究心肌搏动的主要传感技术包括电阻抗传感（EIS）和石英晶体微天平（QCM）。虽然电阻抗技术是研究心肌搏动的金标准，但药物的临床应用也需要实时跟踪，因此，QCM 技术需要进一步发展，成为一种非常重要的质量传感器技术。此外，EIS 与 QCM 结合应用于监测心肌搏动的报道较少。本文通过优化大鼠原代心肌细胞的一系列细胞生长和黏附条件，利用 QCM 与 EIS 相结合，实时监测细胞的黏附和心肌搏动能力。此外，用正性肌力药物异丙肾上腺素（ISO）和负性肌力药物维拉帕米（VRP）处理黏附在 QCM 和 EIS 上的心肌细胞。然后，计算细胞指数（CI）-时间（T）图、搏动幅度（BA）和搏动率（BR），并评估给药前后这些参数的变化。结果表明，QCM 技术的结果不仅与 EIS 获得的结果一致，而且 QCM 技术在计算心肌细胞搏动方面具有一定的灵敏度。因此，我们的研究结果验证了 QCM 技术测量心肌细胞搏动和药物测试的可靠性和有效性。我们希望进一步的研究能够评估 QCM 技术在临床应用中的应用。
