Institute of Electronic and Sensor Materials, Faculty of Materials Science and Materials Technology, Technische Universität Bergakademie Freiberg, 09599 Freiberg, Germany.
Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah 6715847141, Iran.
Biosensors (Basel). 2023 Feb 13;13(2):262. doi: 10.3390/bios13020262.
Gallic acid (GA) is one of the most important polyphenols, being widely used in the food, cosmetic, and pharmaceutical industries due to its biological effects such as antioxidant, antibacterial, anticancer, antiviral, anti-inflammatory, and cardioprotective properties. Hence, simple, fast, and sensitive determination of GA is of particular importance. Considering the fact that GA is an electroactive compound, electrochemical sensors offer great potential for GA quantitation due to their fast response time, high sensitivity, and ease of use. A simple, fast, and sensitive GA sensor was fabricated on the basis of a high-performance bio-nanocomposite using spongin as a natural 3D polymer, atacamite, and multi-walled carbon nanotubes (MWCNTs). The developed sensor showed an excellent response toward GA oxidation with remarkable electrochemical features due to the synergistic effects of 3D porous spongin and MWCNTs, which provide a large surface area and enhance the electrocatalytic activity of atacamite. At optimal conditions by differential pulse voltammetry (DPV), a good linear relationship was obtained between peak currents and GA concentrations in a wild linear range of 500 nM to 1 mM. Subsequently, the proposed sensor was used to detect GA in red wine as well as in green and black tea, confirming its great potential as a reliable alternative to conventional methods for GA determination.
没食子酸（GA）是最重要的多酚之一，由于其具有抗氧化、抗菌、抗癌、抗病毒、抗炎和心脏保护等生物作用，因此被广泛应用于食品、化妆品和制药行业。因此，简单、快速和灵敏地测定 GA 尤为重要。鉴于 GA 是一种电化学活性化合物，电化学传感器因其响应时间快、灵敏度高、使用方便而具有很大的 GA 定量潜力。本工作基于一种高性能的生物纳米复合材料，以海绵蛋白作为天然的 3D 聚合物、氯铜矿和多壁碳纳米管（MWCNTs），制备了一种简单、快速和灵敏的 GA 传感器。由于 3D 多孔海绵蛋白和 MWCNTs 的协同作用，该开发的传感器对 GA 氧化表现出优异的响应，具有显著的电化学特性，3D 多孔海绵蛋白和 MWCNTs 提供了更大的表面积并增强了氯铜矿的电催化活性。在差分脉冲伏安法（DPV）的最佳条件下，在 500 nM 至 1 mM 的宽线性范围内，峰电流与 GA 浓度之间呈现良好的线性关系。随后，该传感器被用于检测红酒以及绿茶和红茶中的 GA，证实了其作为传统 GA 测定方法的可靠替代品的巨大潜力。
