Yu Ruihan, Wang Lifeng, Ma Yanqiu, Zang Jingnan, Qing Mingmin, Chi Yujie, Chi Yuan
College of Food Science, Northeast Agricultural University, Harbin 150030, China.
College of Engineering, Northeast Agricultural University, Harbin 150030, China.
Foods. 2023 Feb 18;12(4):881. doi: 10.3390/foods12040881.
In this study, differences in the protein content and functional and physicochemical properties of four varieties of egg white (EW) were studied by adding 4-10% sucrose or NaCl and then heating them at 70 °C for 3 min. According to a high-performance liquid chromatography (HPLC) analysis, the percentages of ovalbumin, lysozyme and ovotransferrin rose with an increase in the NaCl or sucrose concentration; however, the percentages of ovomucin and ovomucoid decreased. Furthermore, the foaming properties, gel properties, particle size, α-helixes, β-sheets, sulfhydryl groups and disulfide bond content also increased, whereas the content of β-turns and random coils decreased. In addition, the total soluble protein content and functional and physicochemical properties of black bone (BB) chicken and Gu-shi (GS) EWs were higher than those of Hy-Line brown (HY-LINE) and Harbin White (HW) Ews ( < 0.05). Subsequently, transmission electron microscopy (TEM) confirmed the changes in the EW protein structure in the four varieties of Ews. As the aggregations increased, the functional and physicochemical properties decreased. The protein content and functional and physicochemical properties of Ews after heating were correlated with the concentration of NaCl and sucrose and the EW varieties.
在本研究中,通过添加4%-10%的蔗糖或氯化钠,然后在70℃下加热3分钟,研究了四种蛋清(EW)品种在蛋白质含量以及功能和理化性质方面的差异。根据高效液相色谱(HPLC)分析,随着氯化钠或蔗糖浓度的增加,卵清蛋白、溶菌酶和转铁蛋白的百分比上升;然而,卵粘蛋白和卵类粘蛋白的百分比下降。此外,发泡性能、凝胶性能、粒径、α-螺旋、β-折叠、巯基和二硫键含量也增加,而β-转角和无规卷曲的含量下降。此外,乌骨(BB)鸡和固始(GS)蛋清的总可溶性蛋白含量以及功能和理化性质高于海兰褐(HY-LINE)和哈尔滨白(HW)蛋清(P<0.05)。随后,透射电子显微镜(TEM)证实了四种蛋清品种中蛋清蛋白质结构的变化。随着聚集体增加,功能和理化性质下降。加热后蛋清的蛋白质含量以及功能和理化性质与氯化钠和蔗糖浓度以及蛋清品种相关。