College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450001, China.
Key Laboratory of Livestock and Poultry Resources (Poultry) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Zhengzhou 450001, China.
Genes (Basel). 2023 Jan 28;14(2):339. doi: 10.3390/genes14020339.
A growing number of studies have shown that members of the ankyrin repeat and suppressors of cytokine signaling (SOCS) box-containing protein (ASB) family are extensively involved in biological processes such as cell growth, tissue development, insulin signaling, ubiquitination, protein degradation, and skeletal muscle membrane protein formation, while the specific biological role of ankyrin-repeat and SOCS box protein 9 () remains unclear. In this study, a 21 bp indel in the intron of was identified for the first time in 2641 individuals from 11 different breeds and an F resource population, and differences were observed among individuals with different genotypes (II, ID, and DD). An association study of a cross-designed F resource population revealed that the 21-bp indel was significantly related to growth and carcass traits. The significantly associated growth traits were body weight (BW) at 4, 6, 8, 10, and 12 weeks of age; sternal length (SL) at 4, 8, and 12 weeks of age; body slope length (BSL) at 4, 8, and 12 weeks of age; shank girth (SG) at 4 and 12 weeks of age; tibia length (TL) at 12 weeks of age; and pelvic width (PW) at 4 weeks of age ( < 0.05). This indel was also significantly correlated with carcass traits including semievisceration weight (SEW), evisceration weight (EW), claw weight (CLW), breast muscle weight (BMW), leg weight (LeW), leg muscle weight (LMW), claw rate (CLR), and shedding weight (ShW) ( < 0.05). In commercial broilers, the II genotype was the dominant genotype and underwent extensive selection. Interestingly, the gene was expressed at significantly higher levels in the leg muscles of Arbor Acres broilers than those of Lushi chickens, while the opposite was true for the breast muscles. In summary, the 21-bp indel in the gene significantly influenced the expression of the gene in muscle tissue and was associated with multiple growth and carcass traits in the F resource population. These findings suggested that the 21-bp indel within the gene could be used in marker-assisted selection breeding for traits related to chicken growth.
越来越多的研究表明，锚蛋白重复和细胞因子信号抑制物（SOCS）盒蛋白（ASB）家族成员广泛参与细胞生长、组织发育、胰岛素信号、泛素化、蛋白质降解和骨骼肌膜蛋白形成等生物学过程，而锚蛋白重复和 SOCS 盒蛋白 9 () 的具体生物学作用尚不清楚。在这项研究中，首次在来自 11 个不同品种和 F 资源群体的 2641 个人中鉴定出 基因内含子中的 21bp 缺失/插入，并且观察到不同基因型（II、ID 和 DD）个体之间存在差异。F 资源群体的交叉设计关联研究表明，21bp 缺失/插入与生长和胴体性状显著相关。与生长性状显著相关的是 4、6、8、10 和 12 周龄时的体重（BW）；4、8 和 12 周龄时的胸骨长度（SL）；4、8 和 12 周龄时的体斜长（BSL）；4 和 12 周龄时的胫骨长度（TL）；4 周龄时的骨盆宽度（PW）（<0.05）。该缺失/插入也与半净膛重（SEW）、全净膛重（EW）、爪重（CLW）、胸肌重（BMW）、腿重（LeW）、腿肌重（LMW）、爪率（CLR）和脱落重（ShW）等胴体性状显著相关（<0.05）。在商品肉鸡中，II 基因型是优势基因型，并经历了广泛的选择。有趣的是，Arbor Acres 肉鸡的腿部肌肉中 基因的表达水平显著高于鹿石鸡，而在胸肌中则相反。总之，基因内含子中的 21bp 缺失/插入显著影响肌肉组织中基因的表达，并与 F 资源群体中的多个生长和胴体性状相关。这些发现表明，基因内的 21bp 缺失/插入可用于与鸡生长相关性状的标记辅助选择育种。
