Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
Department of Parasitology, National Institute of Infectious Diseases, Tokyo 113-0033, Japan.
Genes (Basel). 2023 Jan 31;14(2):379. doi: 10.3390/genes14020379.
is the enteric protozoan parasite responsible for amebiasis. Trophozoites of ingest human cells in the intestine and other organs, which is the hallmark of its pathogenesis. Phagocytosis and trogocytosis are pivotal biological functions for its virulence and also contribute to the proliferation of nutrient uptake from the environment. We previously elucidated the role of a variety of proteins associated with phagocytosis and trogocytosis, including Rab small GTPases, Rab effectors, including retromer, phosphoinositide-binding proteins, lysosomal hydrolase receptors, protein kinases, and cytoskeletal proteins. However, a number of proteins involved in phagocytosis and trogocytosis remain to be identified, and mechanistic details of their involvement must be elucidated at the molecular level. To date, a number of studies in which a repertoire of proteins associated with phagosomes and potentially involved in phagocytosis have been conducted. In this review, we revisited all phagosome proteome studies we previously conducted in order to reiterate information on the proteome of phagosomes. We demonstrated the core set of constitutive phagosomal proteins and also the set of phagosomal proteins recruited only transiently or in condition-dependent fashions. The catalogs of phagosome proteomes resulting from such analyses can be a useful source of information for future mechanistic studies as well as for confirming or excluding a possibility of whether a protein of interest in various investigations is likely or is potentially involved in phagocytosis and phagosome biogenesis.
是引起阿米巴病的肠道原生动物寄生虫。滋养体在肠道和其他器官中摄取人体细胞，这是其发病机制的标志。吞噬作用和胞饮作用是其毒力的关键生物学功能，也有助于从环境中摄取营养物质的增殖。我们之前阐明了与吞噬作用和胞饮作用相关的多种蛋白质的作用，包括 Rab 小分子 GTP 酶、Rab 效应物（包括逆行转运体）、磷酸肌醇结合蛋白、溶酶体水解酶受体、蛋白激酶和细胞骨架蛋白。然而，仍有许多参与吞噬作用和胞饮作用的蛋白质有待鉴定，并且必须在分子水平上阐明它们参与的机制细节。迄今为止，已经进行了许多研究，其中涉及与吞噬体相关并可能参与吞噬作用的一系列蛋白质。在这篇综述中，我们重新审视了我们之前进行的所有与吞噬体相关的蛋白质组学研究，以重申有关吞噬体蛋白质组的信息。我们展示了组成性吞噬体蛋白的核心集，以及仅短暂或条件依赖性募集的吞噬体蛋白集。此类分析产生的吞噬体蛋白质组目录可以成为未来机制研究的有用信息来源，以及用于确认或排除各种研究中感兴趣的蛋白质是否可能或可能参与吞噬作用和吞噬体发生。
