Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
Genes (Basel). 2023 Feb 10;14(2):459. doi: 10.3390/genes14020459.
Guanylate cyclase (, ) is a key enzyme in organisms, catalyzing the synthesis of from , thus making work. plays a vital role in the regulation of cell and biological growth as a second messenger in signaling pathways. In this study, we screened and identified from the razor clam , which encoded 1257 amino acids and was widely expressed in different tissues, especially the gill and liver. We also screened one double-stranded RNA (dsRNA), , which was used to knockdown at three larval metamorphosis development stages: trochophores-veliger larve, veliger larve-umbo larve, and umbo larve-creeping larvae. We showed that interference at these stages significantly inhibited larval metamorphosis and survival rates. knockdown resulted in an average metamorphosis rate of 60% and an average mortality rate of 50% when compared with control clams. After 50 days, shell length and body weight were inhibited to 53% and 66%, respectively. Thus, appeared to regulate metamorphosis development and growth in By examining the role of the key gene in the metamorphosis development of larvae and the growth and development period, we can provide some data reference for studying the growth and development mechanism of shellfish, and the results provided basic information for the breeding of
鸟苷酸环化酶（，）是生物体中的一种关键酶，催化从生成，从而使发挥作用。作为信号通路中的第二信使，在细胞和生物生长的调节中起着至关重要的作用。在本研究中，我们从刀蛤中筛选并鉴定了，其编码 1257 个氨基酸，广泛表达于不同组织，尤其是鳃和肝脏。我们还筛选出一种双链 RNA（dsRNA），用于在三个幼虫变态发育阶段干扰：担轮幼虫-面盘幼虫、面盘幼虫-耳状幼虫和耳状幼虫-匍匐幼虫。结果表明，这些阶段的干扰显著抑制幼虫变态和存活率。与对照蛤相比， 干扰导致幼虫平均变态率为 60%，平均死亡率为 50%。50 天后，壳长和体重分别抑制至 53%和 66%。因此，在中似乎调节变态发育和生长。通过研究关键基因在幼虫变态发育和生长发育阶段的作用，可为研究贝类生长发育机制提供一些数据参考，为贝类的养殖提供基础信息。
