Department of Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, MOA Laboratory of Animal Genetics and Breeding, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Jiang Xi Province Key Lab of Genetic Improvement of Indigenous Chicken Breeds, Institution of Biological Technology, Nanchang Normal University, Nanchang 330029, China.
Genes (Basel). 2023 Feb 17;14(2):511. doi: 10.3390/genes14020511.
The screening of important candidate genes and the identification of genetic markers are important for molecular selection in the pig industry. The hematopoietically expressed homeobox () gene plays an important role in embryonic development and organogenesis; however, the genetic variation and expression pattern of the porcine gene remains to be clarified. In this study, semiquantitative RT-PCR and immunohistochemistry results showed the specific expression of the gene in porcine cartilage tissues. A novel haplotype consisting of two SNPs rs80901185 (T > C) and rs80934526 (A > G) was detected in the promoter region of the gene. The expression of the gene was significantly higher in Yorkshire pigs (TA haplotype) than in Wuzhishan pigs (CG haplotype), and a population analysis showed that this haplotype was significantly associated with body length. An analysis subsequently revealed that the -586 to -1 bp region of the gene promoter showed the highest activity. Furthermore, we found that the activity of the TA haplotype was significantly higher than that of the CG haplotype by changing the potential binding of transcription factors YY1 and HDAC2. In summary, we conclude that the porcine gene may contribute to the breeding of pigs for body length traits.
筛选重要的候选基因和鉴定遗传标记对于猪产业的分子选择很重要。造血表达同源盒（HOX）基因在胚胎发育和器官发生中起着重要作用；然而，猪 HOX 基因的遗传变异和表达模式仍有待阐明。在这项研究中，半定量 RT-PCR 和免疫组织化学结果表明，基因在猪软骨组织中特异性表达。在基因启动子区域检测到一个由两个 SNP(rs80901185[T>C]和 rs80934526[A>G])组成的新单倍型。与五指山猪(CG 单倍型)相比，约克夏猪(TA 单倍型)的基因表达显著升高，群体分析表明该单倍型与体长显著相关。进一步分析表明，基因启动子的-586 至-1 bp 区域具有最高的活性。此外，我们发现通过改变转录因子 YY1 和 HDAC2 的潜在结合，TA 单倍型的活性明显高于 CG 单倍型。综上所述，我们得出结论，猪基因可能有助于体长性状的猪种选育。
