Wei Dawei, Raza Sayed Haidar Abbas, Zhang Jiupan, Gui Linsheng, Rahman Siddiq Ur, Khan Rajwali, Hosseini Seyed Mahdi, Kaleri Hubdar Ali, Zan Linsen
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China; National Beef Cattle Improvement Center of Northwest A&F University, Yangling 712100, China.
Guyuan Branch of Ningxia Academy of Agriculture and Forestry Sciences, 756000 Guyuan, China.
Gene. 2018 May 20;656:9-16. doi: 10.1016/j.gene.2018.02.059. Epub 2018 Feb 26.
The sine oculis homeobox homolog 4 (SIX4) gene belongs to the SIX gene family, which plays a critical role in muscle regeneration and early stages of ontogeny. This study aimed to detect promoter variations of bovine SIX4 genes in Qinchuan cattle, and to evaluate the effect of transcription regulations and body measurement traits. Quantitative real-time PCR (qPCR) results showed that the mRNA expression levels of SIX4 gene were found significantly highest in longissimus thoracis tissue and individual before attaining the stage of physiological maturity. Using sequencing technology on a total of 428 Qinchuan cattle, seven single nucleotide polymorphisms (SNPs) were identified in the promoter region of SIX4, and seven haplotypes representing 18 potential transcription factor compositions of polymorphic potential cis-acting elements. Association analysis indicated that the H-H diplotype performed greater withers height, chest depth, chest circumference, back fat thickness and ultrasound loin muscle area (P < 0.05) than H-H, which were consistent with the promoter activity of Hap3 haplotype was higher than the Hap5 and Hap6 haplotype in vitro. These potential transcription factor information and combined genotypes H-H of the SIX4 gene can be used as a molecular marker for selection of economic traits in Qinchuan cattle.
眼无同源框4（SIX4）基因属于SIX基因家族，该家族在肌肉再生和个体发育早期阶段发挥着关键作用。本研究旨在检测秦川牛中牛SIX4基因的启动子变异，并评估转录调控和体尺性状的影响。定量实时PCR（qPCR）结果显示，SIX4基因的mRNA表达水平在生理成熟阶段之前，在胸最长肌组织和个体中显著最高。对总共428头秦川牛使用测序技术，在SIX4的启动子区域鉴定出7个单核苷酸多态性（SNP），以及代表多态性潜在顺式作用元件的18种潜在转录因子组成的7种单倍型。关联分析表明，H-H双倍型在肩高、胸深、胸围、背膘厚度和超声腰大肌面积方面比H-H表现更好（P<0.05），这与体外Hap3单倍型的启动子活性高于Hap5和Hap6单倍型一致。这些潜在的转录因子信息和SIX4基因的组合基因型H-H可作为秦川牛经济性状选择的分子标记。
