Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370146, Chile.
Interdisciplinary Center for Aquaculture Research (INCAR), Concepción 4030000, Chile.
Genes (Basel). 2023 Feb 17;14(2):512. doi: 10.3390/genes14020512.
In aquaculture, many stressors can negatively affect growth in teleosts. It is believed that cortisol performs glucocorticoid and mineralocorticoid functions because teleosts do not synthesize aldosterone. However, recent data suggest that 11-deoxycorticosterone (DOC) released during stress events may be relevant to modulate the compensatory response. To understand how DOC modifies the skeletal muscle molecular response, we carried out a transcriptomic analysis. Rainbow trout () were intraperitoneally treated with physiological doses of DOC in individuals pretreated with mifepristone (glucocorticoid receptor antagonist) or eplerenone (mineralocorticoid receptor antagonist). RNA was extracted from the skeletal muscles, and cDNA libraries were constructed from vehicle, DOC, mifepristone, mifepristone plus DOC, eplerenone, and eplerenone plus DOC groups. The RNA-seq analysis revealed 131 differentially expressed transcripts (DETs) induced by DOC with respect to the vehicle group, mainly associated with muscle contraction, sarcomere organization, and cell adhesion. In addition, a DOC versus mifepristone plus DOC analysis revealed 122 DETs related to muscle contraction, sarcomere organization, and skeletal muscle cell differentiation. In a DOC versus eplerenone plus DOC analysis, 133 DETs were associated with autophagosome assembly, circadian regulation of gene expression, and regulation of transcription from RNA pol II promoter. These analyses indicate that DOC has a relevant function in the stress response of skeletal muscles, whose action is differentially modulated by GR and MR and is complementary to cortisol.
在水产养殖中，许多应激源会对硬骨鱼类的生长产生负面影响。由于硬骨鱼类不合成醛固酮，因此人们认为皮质醇具有糖皮质激素和盐皮质激素的功能。然而，最近的数据表明，在应激事件中释放的 11-脱氧皮质酮（DOC）可能与调节代偿反应有关。为了了解 DOC 如何调节骨骼肌的分子反应，我们进行了转录组分析。我们用生理剂量的 DOC 对预先用米非司酮（糖皮质激素受体拮抗剂）或依普利酮（盐皮质激素受体拮抗剂）处理的虹鳟进行了腹腔内注射。从骨骼肌中提取 RNA，并从载体、DOC、米非司酮、米非司酮加 DOC、依普利酮和依普利酮加 DOC 组构建 cDNA 文库。RNA-seq 分析显示，DOC 相对于载体组诱导了 131 个差异表达转录本（DETs），主要与肌肉收缩、肌节组织和细胞黏附有关。此外，DOC 与米非司酮加 DOC 的分析显示，有 122 个 DET 与肌肉收缩、肌节组织和骨骼肌细胞分化有关。在 DOC 与依普利酮加 DOC 的分析中，有 133 个 DET 与自噬体组装、基因表达的昼夜节律调节和 RNA pol II 启动子转录的调节有关。这些分析表明，DOC 在骨骼肌的应激反应中具有重要功能，其作用受 GR 和 MR 的差异调节，并与皮质醇互补。
