Laboratory Department, Children's Hospital of Soochow University, Suzhou 215025, China.
Department of Medical Microbiology, School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou 215127, China.
Genes (Basel). 2023 Feb 18;14(2):520. doi: 10.3390/genes14020520.
() bloodstream infections (BSIs) are among the most predominant causes of death in infants and children worldwide. NDM-5 (New Delhi Metallo-lactamase-5) is responsible for one of the main mechanisms of carbapenem resistance in . To analyze the phenotypic and genomic characteristics of NDM-5-producing from bloodstream infections (BSIs), a total of 114 strains was collected from a children's hospital in Jiangsu province, China. Eight -carrying strains were identified which were all carbapenem-resistant and carried diverse antimicrobial resistance genes apart from . They belonged to six distinct sequence types (STs) and serotypes including one each for ST38/O7:H8, ST58/O?:H37, ST131/O25:H4, ST156/O11:H25 and ST361/O9:H30 and three strains are originating from a single clone belonging to ST410/O?:H9. Apart from , the strains isolated from BSIs also carried other β-lactamase genes, including ( = 4), ( = 2), ( = 3), ( = 1), ( = 4) and ( = 5). The genes were located on three different types of plasmids, which were IncFII/I1 ( = 1), IncX3 ( = 4) and IncFIA/FIB/FII/Q1 ( = 3). The former two types were conjugatively transferable at frequencies of 10 and 10, respectively. The dissemination of NDM-producing strains, which exhibit resistance to the last-line antibiotics, carbapenems, may increase the muti-antimicrobial resistance burden among BSIs and further threaten public health.
（）血流感染（BSIs）是全球婴儿和儿童死亡的主要原因之一。NDM-5（新德里金属β-内酰胺酶-5）是导致碳青霉烯类耐药的主要机制之一。为了分析血流感染（BSIs）中产 NDM-5 的 的表型和基因组特征，从中国江苏省的一家儿童医院共收集了 114 株 。鉴定出 8 株携带 株，均对碳青霉烯类耐药，除 外，还携带多种抗菌药物耐药基因。它们属于 6 个不同的序列类型（STs）和血清型，包括 ST38/O7：H8、ST58/O：H37、ST131/O25：H4、ST156/O11：H25 和 ST361/O9：H30 各 1 株，以及来自于属于 ST410/O：H9 的单一克隆的 3 株。除 外，BSIs 分离的 株还携带其他β-内酰胺酶基因，包括 （=4）、（=2）、（=3）、（=1）、（=4）和 （=5）。 基因位于 3 种不同类型的质粒上，分别为 IncFII/I1（=1）、IncX3（=4）和 IncFIA/FIB/FII/Q1（=3）。前两种类型的可接合转移频率分别为 10 和 10。产 NDM 株的传播，对最后一线抗生素碳青霉烯类耐药，可能会增加 BSIs 中多药耐药的负担，进一步威胁公共健康。
