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Developmentally regulated expression of a truncated myosin light-chain 1F/3F gene.

作者信息

Garfinkel L I, Davidson N

机构信息

Division of Chemistry, California Institute of Technology, Pasadena 91125.

出版信息

Mol Cell Biol. 1987 Oct;7(10):3826-9. doi: 10.1128/mcb.7.10.3826-3829.1987.

DOI:10.1128/mcb.7.10.3826-3829.1987
PMID:3683399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368041/
Abstract

Fast skeletal muscle myosin light-chain I (MLC1f) and myosin light-chain 3 (MLC3f) mRNAs are both derived from a single rat MLC1/3f gene. MLC1f mRNA begins at the first exon of the gene, while MLC3f mRNA begins with exon 2, 10 kilobases downstream. Both mRNAs require alternate splicing of internal exons for accurate expression. We showed that a truncated rat MLC1f/3f gene lacking exon 1 and the first 6.3 kilobases of the intron separating exons 1 and 2 produced rat MLC3f mRNA in a developmentally regulated manner after introduction into myogenic mouse cells, thus demonstrating in vivo the presence of a functional promoter associated with exon 2. Correctly spliced mRNA was produced after transfer of this truncated gene into both myogenic and nonmyogenic cells, indicating that the pattern of splicing of this complex transcript was due to a structural features of the RNA and was independent of cell type.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/004f/368041/9ca5455e6460/molcellb00082-0468-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/004f/368041/6132717b6e49/molcellb00082-0467-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/004f/368041/9c1eb14a6555/molcellb00082-0468-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/004f/368041/9ca5455e6460/molcellb00082-0468-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/004f/368041/6132717b6e49/molcellb00082-0467-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/004f/368041/9c1eb14a6555/molcellb00082-0468-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/004f/368041/9ca5455e6460/molcellb00082-0468-b.jpg

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Developmentally regulated expression of a truncated myosin light-chain 1F/3F gene.
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2
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引用本文的文献

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本文引用的文献

1
Regulated expression of human growth hormone genes in mouse cells.人生长激素基因在小鼠细胞中的调控表达。
Cell. 1982 Jun;29(2):623-31. doi: 10.1016/0092-8674(82)90178-7.
2
Ovalbumin is synthesized in mouse cells transformed with the natural chicken ovalbumin gene.卵清蛋白是在被天然鸡卵清蛋白基因转化的小鼠细胞中合成的。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):244-8. doi: 10.1073/pnas.77.1.244.
3
Alternative transcription and two modes of splicing results in two myosin light chains from one gene.可变转录和两种剪接模式导致一个基因产生两种肌球蛋白轻链。
心肌肌钙蛋白T前体mRNA可变剪接的顺式作用元件要求
Nucleic Acids Res. 1988 Sep 12;16(17):8443-65. doi: 10.1093/nar/16.17.8443.
4
Expression of the troponin complex genes: transcriptional coactivation during myoblast differentiation and independent control in heart and skeletal muscles.肌钙蛋白复合体基因的表达：成肌细胞分化过程中的转录共激活以及心脏和骨骼肌中的独立调控
Mol Cell Biol. 1988 Oct;8(10):4134-42. doi: 10.1128/mcb.8.10.4134-4142.1988.
5
Nucleotide substitutions within the cardiac troponin T alternative exon disrupt pre-mRNA alternative splicing.心肌肌钙蛋白T可变外显子内的核苷酸替换会破坏前体mRNA的可变剪接。
Nucleic Acids Res. 1989 Oct 11;17(19):7905-21. doi: 10.1093/nar/17.19.7905.
6
Myosin light-chain 1/3 gene alternative splicing: cis regulation is based upon a hierarchical compatibility between splice sites.肌球蛋白轻链1/3基因的可变剪接：顺式调控基于剪接位点之间的层级兼容性。
Mol Cell Biol. 1990 May;10(5):2133-44. doi: 10.1128/mcb.10.5.2133-2144.1990.
Nature. 1984;308(5957):333-8. doi: 10.1038/308333a0.
4
Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.导入小鼠成肌细胞和非成肌细胞的鸡肌动蛋白基因的表达与调控
Proc Natl Acad Sci U S A. 1984 May;81(10):2980-4. doi: 10.1073/pnas.81.10.2980.
5
Stable transformation of mouse L cells for human membrane T-cell differentiation antigens, HLA and beta 2-microglobulin: selection by fluorescence-activated cell sorting.利用荧光激活细胞分选技术对人膜T细胞分化抗原、HLA和β2-微球蛋白进行小鼠L细胞的稳定转化：筛选
Proc Natl Acad Sci U S A. 1983 Jan;80(2):524-8. doi: 10.1073/pnas.80.2.524.
6
Cloning and characterization of cDNA sequences corresponding to myosin light chains 1, 2, and 3, troponin-C, troponin-T, alpha-tropomyosin, and alpha-actin.与肌球蛋白轻链1、2和3、肌钙蛋白C、肌钙蛋白T、α-原肌球蛋白和α-肌动蛋白相对应的cDNA序列的克隆与特性分析。
J Biol Chem. 1982 Sep 25;257(18):11078-86.
7
Fast skeletal muscle myosin light chains 1 and 3 are produced from a single gene by a combined process of differential RNA transcription and splicing.快速骨骼肌肌球蛋白轻链1和3由单个基因通过差异RNA转录和剪接的联合过程产生。
J Biol Chem. 1984 Nov 10;259(21):13595-604.
8
A single locus in the mouse encodes both myosin light chains 1 and 3, a second locus corresponds to a related pseudogene.小鼠中的一个基因座编码肌球蛋白轻链1和3，另一个基因座对应一个相关的假基因。
Cell. 1984 Nov;39(1):129-40. doi: 10.1016/0092-8674(84)90198-3.
9
Infectious and selectable retrovirus containing an inducible rat growth hormone minigene.含有可诱导大鼠生长激素小基因的传染性和可选择逆转录病毒。
Science. 1984 Sep 7;225(4666):993-8. doi: 10.1126/science.6089340.
10
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J Cell Biol. 1974 May;61(2):398-413. doi: 10.1083/jcb.61.2.398.