含有可诱导大鼠生长激素小基因的传染性和可选择逆转录病毒。

Infectious and selectable retrovirus containing an inducible rat growth hormone minigene.

作者信息

Miller A D, Ong E S, Rosenfeld M G, Verma I M, Evans R M

出版信息

Science. 1984 Sep 7;225(4666):993-8. doi: 10.1126/science.6089340.

Abstract

A growth hormone minigene carrying its natural promoter (237 nucleotides of chromosomal DNA) was stably propagated in a murine retrovirus containing hypoxanthine-guanine phosphoribosyltransferase as a selectable marker. Glucocorticoid and thyroid hormone inducibility was transferred with the growth hormone gene. Recombinant virus with titers of 10(6) per milliliter was recovered. This demonstration that retroviruses can be used to transfer a nonselectable gene under its own regulatory control enlarges the scope of retroviral vectors as potent tools for gene transfer.

摘要

携带其天然启动子（237个核苷酸的染色体DNA）的生长激素微型基因在含有次黄嘌呤 - 鸟嘌呤磷酸核糖基转移酶作为选择标记的鼠逆转录病毒中稳定增殖。糖皮质激素和甲状腺激素诱导性与生长激素基因一起被转移。回收了滴度为每毫升10(6) 的重组病毒。这一关于逆转录病毒可用于在其自身调控控制下转移非选择基因的证明扩大了逆转录病毒载体作为基因转移有力工具的应用范围。