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电磁辐射通过 TRPV4 和 p-CREB 通路减少 Raw 264.7 细胞的破骨细胞分化。

Reduction of Osteoclastic Differentiation of Raw 264.7 Cells by EMF Exposure through TRPV4 and p-CREB Pathway.

机构信息

Department of Medical Biotechnology, Dongguk University, Goyang-si 10326, Republic of Korea.

出版信息

Int J Mol Sci. 2023 Feb 4;24(4):3058. doi: 10.3390/ijms24043058.

DOI:10.3390/ijms24043058
PMID:36834470
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9959640/
Abstract

In this study, we investigated the effect of EMF exposure on the regulation of RANKL-induced osteoclast differentiation in Raw 264.7 cells. In the EMF-exposed group, the cell volume did not increase despite RANKL treatment, and the expression levels of Caspase-3 remained much lower than those in the RANKL-treated group. TRAP and F-actin staining revealed smaller actin rings in cells exposed to EMF during RANKL-induced differentiation, indicating that EMF inhibited osteoclast differentiation. EMF-irradiated cells exhibited reduced mRNA levels of osteoclastic differentiation markers cathepsin K (CTSK), tartrate-resistant acid phosphatase (TRAP), and matrix metalloproteinase 9 (MMP-9). Furthermore, as measured by RT-qPCR and Western blot, EMF induced no changes in the levels of p-ERK and p-38; however, it reduced the levels of TRPV4 and p-CREB. Overall, our findings indicate that EMF irradiation inhibits osteoclast differentiation through the TRPV4 and p-CREB pathway.

摘要

在这项研究中，我们调查了电磁场暴露对 RANKL 诱导的 Raw 264.7 细胞破骨细胞分化调节的影响。在电磁场暴露组中，尽管有 RANKL 处理，但细胞体积没有增加，并且 Caspase-3 的表达水平仍远低于 RANKL 处理组。TRAP 和 F-肌动蛋白染色显示，在 RANKL 诱导分化过程中暴露于电磁场的细胞中形成的肌动蛋白环较小，表明电磁场抑制了破骨细胞分化。经电磁场照射的细胞中，破骨细胞分化标志物组织蛋白酶 K (CTSK)、抗酒石酸酸性磷酸酶 (TRAP) 和基质金属蛋白酶 9 (MMP-9) 的 mRNA 水平降低。此外，通过 RT-qPCR 和 Western blot 测定，电磁场诱导 p-ERK 和 p-38 水平没有变化；然而，它降低了 TRPV4 和 p-CREB 的水平。总的来说，我们的研究结果表明，电磁场辐射通过 TRPV4 和 p-CREB 途径抑制破骨细胞分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/3f5563ad3f49/ijms-24-03058-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/476c8687342e/ijms-24-03058-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/d8260e90b44b/ijms-24-03058-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/fddcddad058d/ijms-24-03058-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/ea2dda7a276c/ijms-24-03058-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/b614a1c2cef1/ijms-24-03058-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/f52db5584d18/ijms-24-03058-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/3f5563ad3f49/ijms-24-03058-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/476c8687342e/ijms-24-03058-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/d8260e90b44b/ijms-24-03058-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/fddcddad058d/ijms-24-03058-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/ea2dda7a276c/ijms-24-03058-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/b614a1c2cef1/ijms-24-03058-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/f52db5584d18/ijms-24-03058-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da4f/9959640/3f5563ad3f49/ijms-24-03058-g007.jpg

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引用本文的文献

[1]
Promotion of Bone Formation in a Rat Osteoporotic Vertebral Body Defect Model via Suppression of Osteoclastogenesis by Ectopic Embryonic Calvaria Derived Mesenchymal Stem Cells.

Int J Mol Sci. 2024-7-26

[2]
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[3]
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Front Bioeng Biotechnol. 2024-1-24

[4]
The Multifaceted Functions of TRPV4 and Calcium Oscillations in Tissue Repair.

Int J Mol Sci. 2024-1-18

本文引用的文献

[1]
A Vitronectin-Derived Peptide Restores Ovariectomy-Induced Bone Loss by Dual Regulation of Bone Remodeling.

Tissue Eng Regen Med. 2022-12

[2]
Bisphosphonate-based hydrogel mediates biomimetic negative feedback regulation of osteoclastic activity to promote bone regeneration.

Bioact Mater. 2021-11-12

[3]
The Effect of Inflammation on Bone.

Front Physiol. 2021-1-5

[4]
Synergistic effect of electromagnetic fields and nanomagnetic particles on osteogenesis through calcium channels and p-ERK signaling.

J Orthop Res. 2021-8

[5]
Sodium hydrosulfide mitigates dexamethasone-induced osteoblast dysfunction by interfering with mitochondrial function.

Biotechnol Appl Biochem. 2019-6-24

[6]
Zoledronic acid inhibits osteoclast differentiation and function through the regulation of NF-κB and JNK signalling pathways.

Int J Mol Med. 2019-5-23

[7]
Protective Effects of Fermented Oyster Extract against RANKL-Induced Osteoclastogenesis through Scavenging ROS Generation in RAW 264.7 Cells.

Int J Mol Sci. 2019-3-21

[8]
Synergistic effect of rice bran extract and extremely low-frequency electromagnetic fields on dermal papilla/melanocytes in melanogenesis.

Bioelectromagnetics. 2018-12

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Low-intensity pulsed ultrasound inhibits RANKL-induced osteoclast formation via modulating ERK-c-Fos-NFATc1 signaling cascades.

Am J Transl Res. 2018-9-15

[10]
Current imaging techniques in osteoporosis.

Clin Exp Rheumatol. 2018-10-1

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