Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don 344090, Russia.
Moscow Clinical Scientific Center Named after Loginov MHD, Moscow 111123, Russia.
Int J Mol Sci. 2023 Feb 13;24(4):3741. doi: 10.3390/ijms24043741.
Benzo[a]pyrene (BaP) is noted as one of the main cancer-causing pollutants in human beings and may damage the development of crop plants. The present work was designed to explore more insights into the toxic effects of BaP on L. at various doses (20, 40, and 60 MPC) spiked in Haplic Chernozem. A dose-dependent response in phytotoxicity were noted, especially in the biomass of the roots and shoots, at doses of 40 and 60 MPC BaP and the accumulation of BaP in tissues. Physiological and biochemical response indices were severely damaged based on applied doses of BaP. During the histochemical analysis of the localization of superoxide in the leaves of , formazan spots were detected in the area near the leaf's veins. The results of a significant increase in malondialdehyde (MDA) from 2.7 to 5.1 times, proline 1.12- to 2.62-folds, however, a decrease in catalase (CAT) activity was recorded by 1.8 to 1.1 times. The activity of superoxide dismutase (SOD) increased from 1.4 to 2, peroxidase (PRX) from 2.3 to 5.25, ascorbate peroxidase (APOX) by 5.8 to 11.5, glutathione peroxidase (GP) from 3.8 to 7 times, respectively. The structure of the tissues of the roots and leaves of in the variants with BaP changed depending on the dose: it increased the intercellular space, cortical layer, and the epidermis, and the structure of the leaf tissues became looser.
苯并[a]芘（BaP）被认为是人类主要的致癌污染物之一，可能会损害农作物的生长发育。本研究旨在深入探讨 BaP 在不同浓度（20、40 和 60 MPC）下对 L. 的毒害作用。结果表明，在 40 和 60 MPC BaP 处理下，植物的根和地上部分的生物量以及 BaP 在组织中的积累均呈现出剂量依赖性的毒性效应，同时，生理生化响应指标也受到了严重的破坏。在对叶片中超氧化物定位的组织化学分析中，在叶脉附近的区域检测到了甲臜斑点。丙二醛（MDA）含量显著增加了 2.7 至 5.1 倍，脯氨酸增加了 1.12 至 2.62 倍，而过氧化氢酶（CAT）活性则降低了 1.8 至 1.1 倍。超氧化物歧化酶（SOD）活性增加了 1.4 至 2 倍，过氧化物酶（PRX）增加了 2.3 至 5.25 倍，抗坏血酸过氧化物酶（APOX）增加了 5.8 至 11.5 倍，谷胱甘肽过氧化物酶（GP）增加了 3.8 至 7 倍。在添加 BaP 的处理组中，根和叶片的组织结构发生了变化，细胞间隙、皮层和表皮均增大，叶片组织的结构变得更加疏松。
