Department of Nuclear Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 97002, Taiwan.
School of Medicine, College of Medicine, Tzu Chi University, Hualien 97004, Taiwan.
Int J Mol Sci. 2023 Feb 16;24(4):3988. doi: 10.3390/ijms24043988.
Radiogenomic heterogeneity features in F-fluorodeoxyglucose positron emission tomography (F-FDG PET) have become popular in non-small cell lung cancer (NSCLC) research. However, the reliabilities of genomic heterogeneity features and of PET-based glycolytic features in different image matrix sizes have yet to be thoroughly tested. We conducted a prospective study with 46 NSCLC patients to assess the intra-class correlation coefficient (ICC) of different genomic heterogeneity features. We also tested the ICC of PET-based heterogeneity features from different image matrix sizes. The association of radiogenomic features with clinical data was also examined. The entropy-based genomic heterogeneity feature (ICC = 0.736) is more reliable than the median-based feature (ICC = -0.416). The PET-based glycolytic entropy was insensitive to image matrix size change (ICC = 0.958) and remained reliable in tumors with a metabolic volume of <10 mL (ICC = 0.894). The glycolytic entropy is also significantly associated with advanced cancer stages ( = 0.011). We conclude that the entropy-based radiogenomic features are reliable and may serve as ideal biomarkers for research and further clinical use for NSCLC.
氟代脱氧葡萄糖正电子发射断层扫描（F-FDG PET）的放射基因组异质性特征在非小细胞肺癌（NSCLC）研究中已经很流行。然而，基因组异质性特征和不同图像矩阵大小的基于 PET 的糖酵解特征的可靠性尚未得到彻底测试。我们对 46 名 NSCLC 患者进行了一项前瞻性研究，以评估不同基因组异质性特征的组内相关系数（ICC）。我们还测试了来自不同图像矩阵大小的基于 PET 的异质性特征的 ICC。还检查了放射基因组特征与临床数据的相关性。基于熵的基因组异质性特征（ICC=0.736）比基于中位数的特征（ICC=-0.416）更可靠。基于 PET 的糖酵解熵对图像矩阵大小变化不敏感（ICC=0.958），并且在代谢体积<10mL 的肿瘤中仍然可靠（ICC=0.894）。糖酵解熵也与晚期癌症阶段显著相关（=0.011）。我们得出结论，基于熵的放射基因组特征是可靠的，可能是 NSCLC 研究和进一步临床应用的理想生物标志物。
