Complementation of an Eisosomal Yeast 1 Mutant and Characteristics of Eisosomal Distribution in Hyphae of Germinating from Two Different Spore Types.

Eisosomes are plasma-membrane-associated protein complexes of fungi and algae involved in various cellular processes. The eisosome composition of the budding yeast is well described, but there is a limited number of studies only about eisosomes in filamentous fungi. In our study, we examined the LSP-1 protein (NcLSP1). By complementing a Δ1 mutant strain with 1, we show the functional homology of the NcLSP1 to yeast PIL1 rather than to yeast LSP1 and hereby confirm that the NcLSP1 is an eisosomal core protein and suitable eisosomal marker. The subsequent cloning and expression of the :: reporter gene construct in allowed for a systematical investigation of the characteristics of eisosome formation and distribution in different developmental stages. In , the hyphae germinating from sexual and asexual spores are morphologically identical and have been historically recognized as the same type of cells. Here, we demonstrate the structural differences on the cellular level between the hyphae germinating from sexual and asexual spores.