Mitsuhashi Hiromi, Morikawa Ryota, Noguchi Yoh, Takasu Masako
School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Tokyo 192-0392, Japan.
Life (Basel). 2023 Jan 22;13(2):306. doi: 10.3390/life13020306.
The characteristic shape changes observed in the growth and division of L-form cells have been explained by several theoretical studies and simulations using a vesicle model in which the membrane area increases with time. In those theoretical studies, characteristic shapes such as tubulation and budding were reproduced in a non-equilibrium state, but it was not possible to incorporate deformations that would change the topology of the membrane. We constructed a vesicle model in which the area of the membrane increases using coarse-grained particles and analyzed the changes in the shape of growing membrane by the dissipative particle dynamics (DPD) method. In the simulation, lipid molecules were added to the lipid membrane at regular time intervals to increase the surface area of the lipid membrane. As a result, it was found that the vesicle deformed into a tubular shape or a budding shape depending on the conditions for adding lipid molecules. This suggests that the difference in the place where new lipid molecules are incorporated into the cell membrane during the growth of L-form cells causes the difference in the transformation pathway of L-form cells.
一些理论研究和使用囊泡模型的模拟解释了在L型细胞生长和分裂过程中观察到的特征性形状变化，在该模型中膜面积随时间增加。在那些理论研究中，诸如微管形成和出芽等特征形状在非平衡状态下得以重现，但无法纳入会改变膜拓扑结构的变形。我们构建了一个使用粗粒化粒子使膜面积增加的囊泡模型，并通过耗散粒子动力学（DPD）方法分析了生长中膜形状的变化。在模拟中，以固定的时间间隔向脂质膜添加脂质分子以增加脂质膜的表面积。结果发现，根据添加脂质分子的条件，囊泡会变形为管状或出芽状。这表明在L型细胞生长过程中，新脂质分子掺入细胞膜的位置差异导致了L型细胞转化途径的差异。
