Karal-Ogly Dzhina D, Shumeev Alexander N, Keburiya Viktoria V, Mintel Marina V, Rybtsov Stanislav A
The Research Institute of Medical Primatology, 354383 Sochi, Russia.
Centre for Cell Technology and Immunology, Sirius University of Science and Technology, 354340 Sochi, Russia.
Life (Basel). 2023 Jan 23;13(2):316. doi: 10.3390/life13020316.
Non-anthropoid primates cynomolgus monkeys (), also known as crab-eating macaques, are increasingly used in biomedical and preclinical studies due to their evolutionary proximity to humans, sharing similar diets, infectious and senile diseases. Age-related changes and sexual dimorphism of the immune system of C. monkeys have not been sufficiently characterized in literature, though age and sex differences affect the course of diseases and sensitivity to medications. Aging in C. monkeys is accompanied by an increase in CD3+CD4+CD8+ (DP-T) cells, plasma B-cells, and a decrease in platelets. Erythromyeloid bias has also been noticed in older animals. There was an increase in eosinophils, haematocrit (HCT) and haemoglobin concentration (HGB). Senile decline in the function of the immune system had sex differences. An increase in the number of monocytes, cytotoxic lymphocytes (CTL) and a decrease in the T-helper population were more pronounced in older females. A significant reduction in the number of B-cells and activated T-cells was detected in males only. A moderate correlation with the regression model of aging was established for DP-T, HCT and HGB. The reduction in the B cells count in males and the increase in CTL level in females are moderately correlated with age. Other blood cell populations did not show significant correlations in the regression models due to their high sample variability. The novel cell population CD3-CD20loCD16/CD56+, presumably NK-cells subset, was revealed. This cell population demonstrated an increase trend with age in both sexes. Population-statistical age norms for different sexes for young and very old macaques were established. The blood population clusters associated with sex and immune status in older animals were also identified.
非类人猿灵长类动物食蟹猕猴（ ），也被称为食蟹猴，由于其在进化上与人类接近，具有相似的饮食、传染病和老年疾病，越来越多地用于生物医学和临床前研究。尽管年龄和性别差异会影响疾病进程和对药物的敏感性，但食蟹猴免疫系统的年龄相关变化和性别二态性在文献中尚未得到充分描述。食蟹猴的衰老伴随着CD3 + CD4 + CD8 +（双阳性T细胞）细胞、血浆B细胞增加，血小板减少。在老年动物中也发现了红髓偏向。嗜酸性粒细胞、血细胞比容（HCT）和血红蛋白浓度（HGB）增加。免疫系统功能的衰老性下降存在性别差异。老年雌性中单核细胞、细胞毒性淋巴细胞（CTL）数量增加，辅助性T细胞数量减少更为明显。仅在雄性中检测到B细胞和活化T细胞数量显著减少。双阳性T细胞、血细胞比容和血红蛋白与衰老回归模型存在中度相关性。雄性B细胞计数减少和雌性CTL水平增加与年龄中度相关。由于其他血细胞群体样本变异性高，在回归模型中未显示出显著相关性。发现了新的细胞群体CD3 - CD20loCD16/CD56 +，可能是自然杀伤细胞亚群。该细胞群体在两性中均呈现随年龄增长的趋势。建立了年轻和非常老龄食蟹猴不同性别的群体统计年龄标准。还确定了老年动物中与性别和免疫状态相关的血液群体簇。
