Yang Ming, Wang Maojia, Zhou Ming, Zhang Yifu, Yu Keliang, Wang Tao, Bu Tongliang, Tang Zizhong, Zheng Tianrun, Chen Hui
College of Life Science, Sichuan Agricultural University, Ya'an 625014, China.
YongChuan District Center for Disease Control and Prevention, Chongqing 402160, China.
Life (Basel). 2023 Jan 29;13(2):371. doi: 10.3390/life13020371.
Under dry-hot valley climates, (also known as Jin Long Dan Cao), suffers from nocturnal low-temperature stress (LTS) during winter. Here, to investigate the biological significance of terpenoid metabolism during LTS adaptation, the growth state and terpenoid content of under different LTS were detected, and analyzed with the changes in phytohormone. When subjected to LTS, the results demonstrated that the growth activity of was severely suppressed, while the metabolism activity was smoothly stimulated. Meanwhile, the fluctuation in phytohormone content exhibited three different physiological stages, which are considered the stress response, signal amplification, and stress adaptation. Furthermore, drastic changes occurred in the distribution and accumulation of terpenoids, such as blinin (diterpenoids from MEP) accumulating specifically in leaves and oleanolic acid (triterpenoids from MVA) accumulating evenly and globally. The gene expression of MEP and MVA signal transduction pathways also changes under LTS. In addition, a pharmacological study showed that it may be the ABA-SA crosstalk driven by the LTS signal, that balances the metabolic flux in the MVA and MEP pathways in an individual manner. In summary, this study reveals the different standpoints of ABA and SA, and provides a research foundation for the optimization of the regulation of terpenoid metabolic flux within .
在干热河谷气候下，（又名金龙胆草）在冬季遭受夜间低温胁迫（LTS）。在此，为研究低温胁迫适应过程中萜类代谢的生物学意义，检测了不同低温胁迫下的生长状态和萜类含量，并分析其与植物激素变化的关系。结果表明，遭受低温胁迫时，其生长活性受到严重抑制，而代谢活性则受到平稳刺激。同时，植物激素含量的波动呈现出三个不同的生理阶段，分别被认为是应激反应、信号放大和应激适应。此外，萜类化合物的分布和积累发生了剧烈变化，例如叶中特异性积累的布莱宁（来自MEP途径的二萜）和均匀且广泛积累的齐墩果酸（来自MVA途径的三萜）。低温胁迫下，MEP和MVA信号转导途径的基因表达也发生了变化。此外，药理学研究表明，可能是低温胁迫信号驱动的ABA - SA相互作用，以个体方式平衡了MVA和MEP途径中的代谢通量。总之，本研究揭示了ABA和SA的不同作用观点，并为优化金龙胆草内萜类代谢通量的调控提供了研究基础。
