Lee Hana, Lee Jin-Ho, Kim Doyong, Hwang Donghyun, Lee Minjoo, Chung Halim, Kim Tack-Joong, Kim Han Sung
Department of Biomedical Engineering, Yonsei University, Wonju 26493, Republic of Korea.
Division of Biological Science and Technology, Yonsei University, Wonju 26493, Republic of Korea.
Life (Basel). 2023 Feb 1;13(2):404. doi: 10.3390/life13020404.
Obesity is a disease in which fat is abnormally or excessively accumulated in the body, and many studies have been conducted to overcome it with various techniques. In this study, we evaluated whether micro-current stimulation (MCS) can be applied to prevent obesity by regulating the adipogenesis through 3T3-L1 cells and / mice. To specify the intensity of MCS, Oil Red O staining was conducted with various intensities of MCS. Based on these, subsequent experiments used 200 and 400 μA for the intensity of MCS. The expressions of insulin signaling pathway-related proteins, including phosphorylation of IGF-1 and IR, were decreased in all MCS groups, and in turn, downstream signals such as Akt and ERK were decreased. In addition, MCS reduced the nucleus translocation of PPAR-γ and decreased the protein expression of C/EBP-α. In the / mouse model, MCS reduced body weight gain and abdominal adipose tissue volume. In particular, the concentration of triglycerides in serum was also decreased. Taken together, our findings showed that MCS inhibited lipid accumulation by regulating insulin signaling in 3T3-L1, and it was effective at reducing body weight and adipose tissue volume in / mice. These suggest that MCS may be a useful treatment approach for obesity.
肥胖是一种脂肪在体内异常或过度堆积的疾病，人们已经开展了许多研究，尝试用各种技术来攻克这一问题。在本研究中，我们评估了微电流刺激（MCS）是否可通过3T3-L1细胞和/小鼠调节脂肪生成来预防肥胖。为明确MCS的强度，我们用不同强度的MCS进行了油红O染色。基于此，后续实验中MCS的强度采用200和400 μA。所有MCS组中，胰岛素信号通路相关蛋白的表达均下降，包括IGF-1和IR的磷酸化，相应地，下游信号如Akt和ERK也减少。此外，MCS减少了PPAR-γ的核转位，并降低了C/EBP-α的蛋白表达。在/小鼠模型中，MCS减少了体重增加和腹部脂肪组织体积。特别是，血清中甘油三酯的浓度也降低了。综上所述，我们的研究结果表明，MCS通过调节3T3-L1中的胰岛素信号来抑制脂质积累，并且在降低/小鼠的体重和脂肪组织体积方面有效。这些结果表明，MCS可能是一种治疗肥胖的有效方法。
