Department of Biomedical Engineering, Yonsei University, Wonju 26493, Korea.
Division of Biological Science and Technology, Yonsei University, Wonju 26493, Korea.
Int J Mol Sci. 2021 Apr 22;22(9):4361. doi: 10.3390/ijms22094361.
Recently, a variety of safe and effective non-pharmacological methods have been introduced as new treatments of alopecia. Micro-current electrical stimulation (MCS) is one of them. It is generally known to facilitate cell proliferation and differentiation and promote cell migration and ATP synthesis. This study aimed to investigate the hair growth-promoting effect of MCS on human hair follicle-derived papilla cells (HFDPC) and a telogenic mice model. We examined changes in cell proliferation, migration, and cell cycle progression with MCS-applied HFDPC. The changes of expression of the cell cycle regulatory proteins, molecules related to the PI3K/AKT/mTOR/Fox01 pathway and Wnt/β-catenin pathway were also examined by immunoblotting. Subsequently, we evaluated the various growth factors in developing hair follicles by RT-PCR in MCS-applied (MCS) mice model. From the results, the MCS-applied groups with specific levels showed effects on HFDPC proliferation and migration and promoted cell cycle progression and the expression of cell cycle-related proteins. Moreover, these levels significantly activated the Wnt/β-catenin pathway and PI3K/AKT/mTOR/Fox01 pathway. Various growth factors in developing hair follicles, including Wnts, FGFs, , and except for , significantly increased in MCS-applied mice. Our results may confirm that MCS has hair growth-promoting effect on HFDPC as well as telogenic mice model, suggesting a potential treatment strategy for alopecia.
最近，出现了多种安全有效的非药物治疗方法，作为脱发的新疗法。微电流电刺激（MCS）就是其中之一。它通常被认为可以促进细胞增殖和分化，促进细胞迁移和 ATP 合成。本研究旨在探讨 MCS 对人毛囊乳头细胞（HFDPC）和休止期小鼠模型的促毛发生长作用。我们通过 MCS 处理的 HFDPC 检测细胞增殖、迁移和细胞周期进程的变化。通过免疫印迹法还检查了细胞周期调节蛋白、与 PI3K/AKT/mTOR/Fox01 通路和 Wnt/β-catenin 通路相关的分子的表达变化。随后，我们通过 RT-PCR 评估了 MCS 处理（MCS）小鼠模型中发育中的毛囊中的各种生长因子。结果表明，具有特定水平的 MCS 处理组对 HFDPC 的增殖和迁移有影响，并促进了细胞周期进程和细胞周期相关蛋白的表达。此外，这些水平显著激活了 Wnt/β-catenin 通路和 PI3K/AKT/mTOR/Fox01 通路。在 MCS 处理的小鼠中，包括 Wnts、FGFs、 和 在内的各种发育中的毛囊生长因子显著增加，而 除外。我们的结果可能证实 MCS 对 HFDPC 和休止期小鼠模型具有促毛发生长作用，为脱发提供了一种潜在的治疗策略。
