Teodorczyk-Injeyan Julita A, Khella Heba, Injeyan H Stephen
Graduate Education and Research Programs, Canadian Memorial Chiropractic College, Toronto, ON M2H 3J1, Canada.
Department of Clinical Education, Canadian Memorial Chiropractic College, Toronto, ON M2H 3J1, Canada.
Life (Basel). 2023 Feb 8;13(2):468. doi: 10.3390/life13020468.
The present study explores whether the inflammatory biomarker of sterile inflammation, high mobility box 1 (HMGB1), contributes to the inflammatory/nociceptive pathophysiology that characterizes chronic non-specific low back pain (LBP). Patients with chronic LBP (N = 10, >3 pain score on a 11-point Visual Analogue Scale, VAS) and asymptomatic participants (N = 12) provided peripheral blood (PB) samples. The proportion of classical CD14++ monocytes within PB leukocytes was determined by flow cytometry. The plasma and extracellular HMGB1 levels in unstimulated adherent cell (AC) cultures were measured using specific immunoassays. HMGB1 localization in ACs was assessed by immunofluorescent staining. The relative gene expression levels of tumor necrosis factor α (TNFα), interleukin-1 beta (IL-1β) and HMGB1 were determined by quantitative polymerase chain reaction (qRT-PCR) in relation to the pain intensity (11-point VAS scores) in patients with LBP. The extracellular release of HMGB1 in the LBP patient AC cultures was significantly elevated ( = 0.001) and accompanied by its relocation into the cytoplasm from the nuclei. The number of CD14++ monocytes in the patients' PB was significantly ( = 0.03) reduced, while the HMGB1 plasma levels remained comparable to those of the controls. The mRNA levels of TNFα, IL-1β and HMGB1 were overexpressed relative to the controls and those of HMGB1 and IL-1β were correlated with the VAS scores at a significant level ( = 0.01-0.03). The results suggest that HMGB1 may play an important role in the pathophysiology of chronic non-specific LBP.
本研究探讨无菌性炎症的炎症生物标志物高迁移率族蛋白B1（HMGB1）是否促成了慢性非特异性下腰痛（LBP）所特有的炎症/伤害感受性病理生理学过程。慢性LBP患者（N = 10，在11分视觉模拟量表（VAS）上疼痛评分>3）和无症状参与者（N = 12）提供了外周血（PB）样本。通过流式细胞术测定PB白细胞中经典CD14++单核细胞的比例。使用特异性免疫测定法测量未刺激的贴壁细胞（AC）培养物中的血浆和细胞外HMGB1水平。通过免疫荧光染色评估AC中HMGB1的定位。通过定量聚合酶链反应（qRT-PCR）测定与LBP患者的疼痛强度（11分VAS评分）相关的肿瘤坏死因子α（TNFα）、白细胞介素-1β（IL-1β）和HMGB1的相对基因表达水平。LBP患者AC培养物中HMGB1的细胞外释放显著升高（P = 0.001），并伴随着其从细胞核重新定位到细胞质中。患者PB中CD14++单核细胞的数量显著减少（P = 0.03），而HMGB1血浆水平与对照组相当。TNFα、IL-1β和HMGB1的mRNA水平相对于对照组过表达，并且HMGB1和IL-1β的mRNA水平与VAS评分在显著水平上相关（P = 0.01 - 0.03）。结果表明，HMGB1可能在慢性非特异性LBP的病理生理学中起重要作用。
