吗啡对大鼠中脑导水管周围灰质切片中胆囊收缩素八肽释放的作用：纳洛酮的致敏作用

Morphine action on cholecystokinin octapeptide release from rat periaqueductal grey slices: sensitisation by naloxone.

作者信息

Rattray M, de Belleroche J

机构信息

Department of Biochemistry, Charing Cross & Westminster Hospital Medical School, London, U.K.

出版信息

Neuropeptides. 1987 Aug-Sep;10(2):189-200. doi: 10.1016/0143-4179(87)90021-7.

DOI:10.1016/0143-4179(87)90021-7

PMID:3683773

Abstract

Cholecystokinin (CCK) has potent antinociceptive properties when given either peripherally or centrally. An interaction between opiate and CCK-induced antinociception is indicated as CCK-induced analgesia is potentiated by naloxone. Since CCK cells in Periaqueductal grey (PAG) are known to be sensitive to both noxious stimuli and i.v. morphine, the possibility that the PAG was the site of such an interaction was investigated by an in vitro study of the effects of morphine and naloxone on CCK release in PAG. The K+-evoked release of CCK from tissue slices of PAG was unaffected by a wide range of concentrations of morphine. However, after pretreatment with naloxone (10(-9) M), morphine (10(-7)-10(-6) M) caused a significant, dose dependent attenuation of CCK release (70% inhibition at 10(-6) M). These results suggest that the release of CCK in PAG is modulated by opioid systems.

摘要

胆囊收缩素（CCK）无论是外周给药还是中枢给药都具有强大的抗伤害感受特性。由于纳洛酮可增强CCK诱导的镇痛作用，提示阿片类药物与CCK诱导的抗伤害感受之间存在相互作用。已知中脑导水管周围灰质（PAG）中的CCK细胞对伤害性刺激和静脉注射吗啡均敏感，因此通过体外研究吗啡和纳洛酮对PAG中CCK释放的影响，来探究PAG是否为这种相互作用的部位。PAG组织切片中钾离子诱发的CCK释放不受多种浓度吗啡的影响。然而，在用纳洛酮（10⁻⁹ M）预处理后，吗啡（10⁻⁷ - 10⁻⁶ M）可导致CCK释放显著的、剂量依赖性减弱（10⁻⁶ M时抑制70%）。这些结果表明PAG中CCK的释放受阿片系统调节。