Dahal Sanjeev, Hurst Gregory B, Chourey Karuna, Engle Nancy L, Burdick Leah H, Morrell-Falvey Jennifer L, Tschaplinski Timothy J, Doktycz Mitchel J, Pelletier Dale A
Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA.
Genome Science and Technology Program, University of Tennessee, Knoxville, TN 37996, USA.
Metabolites. 2023 Jan 17;13(2):140. doi: 10.3390/metabo13020140.
GM16 associates with , a model plant in biofuel production. releases abundant phenolic glycosides such as salicin, but GM16 cannot utilize salicin, whereas strains are known to utilize compounds similar to the aglycone moiety of salicin-salicyl alcohol. We propose that the association of to is mediated by another organism (such as OV744) that degrades the glucosyl group of salicin. In this study, we demonstrate that in the - salicin co-culture model, grows by degrading salicin to glucose 6-phosphate and salicyl alcohol which is secreted out and is subsequently utilized by GM16 for its growth. Using various quantitative approaches, we elucidate the individual pathways for salicin and salicyl alcohol metabolism present in and , respectively. Furthermore, we were able to establish that the salicyl alcohol cross-feeding interaction between the two strains on salicin medium is carried out through the combination of their respective individual pathways. The research presents one of the potential advantages of salicyl alcohol release by strains such as , and how phenolic glycosides could be involved in attracting multiple types of bacteria into the microbiome.
GM16与生物燃料生产中的模式植物相关联。该植物会释放出大量酚类糖苷，如柳醇，但GM16无法利用柳醇，而已知某些菌株能够利用与柳醇苷元部分类似的化合物——水杨醇。我们推测，GM16与该植物的关联是由另一种生物（如OV744）介导的，这种生物会降解柳醇的葡萄糖基。在本研究中，我们证明在植物 - 柳醇共培养模型中，植物通过将柳醇降解为6 - 磷酸葡萄糖和水杨醇来生长，水杨醇被分泌出来，随后被GM16利用以实现其生长。我们使用各种定量方法，分别阐明了植物和GM16中存在的柳醇和水杨醇代谢的各自途径。此外，我们能够确定，在柳醇培养基上，两种菌株之间的水杨醇交叉喂养相互作用是通过它们各自的个体途径组合来进行的。该研究展示了像该植物这样的菌株释放水杨醇的潜在优势之一，以及酚类糖苷如何可能参与吸引多种类型的细菌进入该植物的微生物群落。
