Artigas Ramírez María Daniela, Agake Shin-Ichiro, Maeda Masumi, Kojima Katsuhiro, Ohkama-Ohtsu Naoko, Yokoyama Tadashi
Iriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, 870 Uehara, Yaeyama, Taketomi, Okinawa 907-1541, Japan.
Institute of Global Innovation Research, Tokyo University of Agriculture and Technology (TUAT), Saiwai-cho 3-5-8, Fuchu, Tokyo 183-8538, Japan.
Microorganisms. 2023 Jan 17;11(2):232. doi: 10.3390/microorganisms11020232.
Biofertilizers are agricultural materials capable of reducing the usage amounts of chemical fertilizers. Spore-forming microorganisms (SFM) could be used for plant growth promotion or to improve plant health. Until now, biofertilizers based on SFM have been applied for rice and other crops. In this study, we isolated and characterized SFM, which were colonized on the L. roots. SFM were analyzed regarding the short-term effects of biofertilization on the nursery growths. Analysis was performed without nitrogen or any inorganic fertilizer and was divided into two groups, including bacteria and fungi. SF-bacteria were dominated by the group, including species from , , , , and mainly (50%). The fungi group was classified as , , and mainly (80%), with a predominance of and species. In plant performance in comparison with TUAT1, some bacteria and fungus isolates significantly improved the early growth of rice, based on 48 h inoculum with 10 CFU mL. Furthermore, several SFM showed positive physiological responses under abiotic stress or with limited nutrients such as phosphorous (P). Moreover, the metabolic fingerprint was obtained. The biofertilizer based on SFM could significantly reduce the application of the inorganic fertilizer and improve the lodging resistances of rice, interactively enhancing better plant health and crop production.
生物肥料是能够减少化肥使用量的农业材料。形成孢子的微生物（SFM）可用于促进植物生长或改善植物健康。到目前为止，基于SFM的生物肥料已应用于水稻和其他作物。在本研究中，我们分离并鉴定了定殖在水稻根上的SFM。分析了生物施肥对育苗生长的短期影响。在不使用氮肥或任何无机肥料的情况下进行分析，并分为细菌和真菌两组。SF细菌以该组为主，包括来自[具体菌属1]、[具体菌属2]、[具体菌属3]、[具体菌属4]、[具体菌属5]的菌种，主要是[具体菌种]（50%）。真菌组被分类为[具体真菌类别1]、[具体真菌类别2]，主要是[具体真菌种类]（80%），以[具体优势真菌种类1]和[具体优势真菌种类2]为主。与TUAT1相比，在接种10 CFU/mL菌液48小时的情况下，一些细菌和真菌分离株显著促进了水稻的早期生长。此外，几种SFM在非生物胁迫或养分有限（如磷）的情况下表现出积极的生理反应。此外，还获得了代谢指纹图谱。基于SFM的生物肥料可以显著减少无机肥料的施用，提高水稻的抗倒伏能力，从而交互性地增强植物健康和作物产量。
