Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia.
Faculty of Natural sciences, Novosibirsk State University, 630090 Novosibirsk, Russia.
Molecules. 2023 Feb 10;28(4):1695. doi: 10.3390/molecules28041695.
Fluorinated human serum albumin conjugates were prepared and tested as potential metal-free probes for F magnetic resonance imaging (MRI). Each protein molecule was modified by several fluorine-containing compounds via the N-substituted natural acylating reagent homocysteine thiolactone. Albumin conjugates retain the protein's physical and biological properties, such as its 3D dimensional structure, aggregation ability, good solubility, proteolysis efficiency, biocompatibility, and low cytotoxicity. A dual-labeled with cyanine 7 fluorescence dye and fluorine reporter group albumin were synthesized for simultaneous fluorescence imaging and F MRI. The preliminary in vitro studies show the prospects of albumin carriers for multimodal imaging.
氟代人血清白蛋白缀合物被制备并测试为潜在的无金属探针用于 F 磁共振成像 (MRI)。每个蛋白质分子通过 N-取代的天然酰化试剂同型半胱氨酸硫内酯被几种含氟化合物修饰。白蛋白缀合物保留了蛋白质的物理和生物学特性，如三维结构、聚集能力、良好的溶解性、蛋白水解效率、生物相容性和低细胞毒性。合成了一种同时带有菁染料 7 荧光染料和氟报告基团的双标记白蛋白，用于同时进行荧光成像和 F MRI。初步的体外研究表明，白蛋白载体在多模态成像中有应用前景。
