Kudinova Alina, Grishin Alexander, Grunina Tatiana, Poponova Maria, Bulygina Inna, Gromova Maria, Choudhary Rajan, Senatov Fedor, Karyagina Anna
Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Healthcare of the Russian Federation, 123098 Moscow, Russia.
All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, 127550 Moscow, Russia.
Pathogens. 2023 Jan 23;12(2):177. doi: 10.3390/pathogens12020177.
Diopside-based ceramic is a perspective biocompatible material with numerous potential applications in the field of bone prosthetics. Implantable devices and materials are often prone to colonization and biofilm formation by pathogens such as , which in the case of bone grafting leads to osteomyelitis, an infectious bone and bone marrow injury. To lower the risk of bacterial colonization, implanted materials can be impregnated with antimicrobials. In this work, we loaded the antibacterial enzyme lysostaphin on diopside powder and studied the antibacterial and antibiofilm properties of such material to probe the utility of this approach for diopside-based prosthetic materials.
Diopside powder was synthesized by the solid-state method, lysostaphin was loaded on diopside by adsorption, the release of lysostaphin from diopside was monitored by ELISA, and antibacterial and anti-biofilm activity was assessed by standard microbiological procedures.
Lysostaphin released from diopside powder showed high antibacterial activity against planktonic bacteria and effectively destroyed 24-h staphylococcal biofilms. Diopside-based materials possess a potential for the development of antibacterial bone grafting materials.
透辉石基陶瓷是一种具有前景的生物相容性材料，在骨修复领域有众多潜在应用。可植入装置和材料往往容易被诸如[病原体名称未给出]等病原体定植并形成生物膜，在骨移植的情况下会导致骨髓炎，即一种感染性骨和骨髓损伤。为降低细菌定植风险，可在植入材料中浸渍抗菌剂。在本研究中，我们将抗菌酶溶葡萄球菌素负载于透辉石粉末上，并研究了该材料的抗菌和抗生物膜特性，以探究这种方法对透辉石基修复材料的实用性。
采用固态法合成透辉石粉末，通过吸附将溶葡萄球菌素负载于透辉石上，用酶联免疫吸附测定法监测溶葡萄球菌素从透辉石中的释放情况，并通过标准微生物学程序评估抗菌和抗生物膜活性。
从透辉石粉末释放的溶葡萄球菌素对浮游细菌显示出高抗菌活性，并有效破坏了24小时的葡萄球菌生物膜。透辉石基材料具有开发抗菌骨移植材料的潜力。
