Tsamesidis Ioannis, Theocharidou Anna, Beketova Anastasia, Bousnaki Maria, Chatzimentor Iason, Pouroutzidou Georgia K, Gkiliopoulos Dimitrios, Kontonasaki Eleana
Department of Prosthodontics, School of Dentistry, Faculty of Health Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Laboratory of Advanced Materials and Devices (AMDeLab), School of Physics, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
Pharmaceutics. 2023 Feb 15;15(2):655. doi: 10.3390/pharmaceutics15020655.
A promising strategy to enhance bone regeneration is the use of bioactive materials doped with metallic ions with therapeutic effects and their combination with active substances and/or drugs. The aim of the present study was to investigate the osteogenic capacity of human periodontal ligament cells (hPDLCs) in culture with artemisinin (ART)-loaded Ce-doped calcium silicate nanopowders (NPs); Methods: Mesoporous silica, calcium-doped and calcium/cerium-doped silicate NPs were synthesized via a surfactant-assisted cooperative self-assembly process. Human periodontal ligament cells (hPDLCs) were isolated and tested for their osteogenic differentiation in the presence of ART-loaded and unloaded NPs through alkaline phosphatase (ALP) activity and Alizarine red S staining, while their antioxidant capacity was also evaluated; Results: ART promoted further the osteogenic differentiation of hPDLCs in the presence of Ce-doped NPs. Higher amounts of Ce in the ART-loaded NPs inversely affected the mineral deposition process by the hPDLCs. ART and Ce in the NPs have a synergistic role controlling the redox status and reducing ROS production from the hPDLCs; Conclusions: By monitoring the Ce amount and ART concentration, mesoporous NPs with optimum properties can be developed towards bone tissue regeneration demonstrating also potential application in periodontal tissue regeneration strategies.
一种增强骨再生的有前景的策略是使用掺杂有具有治疗作用的金属离子的生物活性材料，以及将它们与活性物质和/或药物相结合。本研究的目的是研究在载有青蒿素（ART）的铈掺杂硅酸钙纳米粉末（NPs）培养条件下人牙周膜细胞（hPDLCs）的成骨能力；方法：通过表面活性剂辅助的协同自组装过程合成介孔二氧化硅、钙掺杂和钙/铈掺杂的硅酸盐纳米颗粒。分离人牙周膜细胞（hPDLCs），并通过碱性磷酸酶（ALP）活性和茜素红S染色检测其在载有和未载有ART的纳米颗粒存在下的成骨分化情况，同时还评估了它们的抗氧化能力；结果：在铈掺杂纳米颗粒存在的情况下，ART进一步促进了hPDLCs的成骨分化。载有ART的纳米颗粒中较高含量的铈对hPDLCs的矿物质沉积过程产生了相反的影响。纳米颗粒中的ART和铈在控制氧化还原状态和减少hPDLCs产生的活性氧方面具有协同作用；结论：通过监测铈含量和ART浓度，可以开发出具有最佳性能的介孔纳米颗粒用于骨组织再生，这也证明了其在牙周组织再生策略中的潜在应用。
