Sim Ji-Eun, Oh Sung-Dug, Kang Kiyoon, Shin Yu-Mi, Yun Doh-Won, Baek So-Hyeon, Choi Yong-Eui, Park Sang-Un, Kim Jae-Kwang
Division of Life Sciences and Convergence Research Center for Insect Vectors, Incheon National University, Incheon 22012, Republic of Korea.
National Institute of Agricultural Sciences, Rural Development Administration (RDA), Wanju-gun 55365, Republic of Korea.
Plants (Basel). 2023 Feb 8;12(4):758. doi: 10.3390/plants12040758.
Event DS rice producing protopanaxadiol (PPD) has been previously developed by inserting dammarenediol-II synthase gene () and PPD synthase gene (). We performed a gas chromatography-mass spectrometry (GC-MS)-based metabolomics of the DS rice to identify metabolic alterations as the effects of genetic engineering by measuring the contents of 65 metabolites in seeds and 63 metabolites in leaves. Multivariate analysis and one-way analysis of variance between DS and non-genetically modified (GM) rice showed that DS rice accumulated fewer tocotrienols, tocopherols, and phytosterols than non-GM rice. These results may be due to competition for the same precursors because PPDs in DS rice are synthesized from the same precursors as those of phytosterols. In addition, multivariate analysis of metabolic data from rice leaves revealed that composition differed by growth stage rather than genetic modifications. Our results demonstrate the potential of metabolomics for identifying metabolic alterations in response to genetic modifications.
此前已通过插入达玛烯二醇-II 合酶基因（）和原人参二醇（PPD）合酶基因（）培育出能产生 PPD 的事件 DS 水稻。我们对 DS 水稻进行了基于气相色谱-质谱联用（GC-MS）的代谢组学分析，通过测量种子中的 65 种代谢物和叶片中的 63 种代谢物含量，来确定作为基因工程效应的代谢变化。DS 水稻与非转基因（GM）水稻之间的多变量分析和单因素方差分析表明，DS 水稻积累的生育三烯酚、生育酚和植物甾醇比非转基因水稻少。这些结果可能是由于对相同前体的竞争，因为 DS 水稻中的 PPD 与植物甾醇由相同的前体合成。此外，对水稻叶片代谢数据的多变量分析表明，其组成因生长阶段而异，而非基因改造。我们的结果证明了代谢组学在识别基因改造引起的代谢变化方面的潜力。
