Wang Zhiquan, Ni Longjie, Liu Liangqin, Yuan Haiyan, Gu Chunsun
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Memorial Sun Yat-Sen), Nanjing 210014, China.
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Nanjing 210014, China.
Plants (Basel). 2023 Feb 12;12(4):823. doi: 10.3390/plants12040823.
Cadmium (Cd) stress has a major impact on ecosystems, so it is important to find suitable Cd-tolerant plants while elucidating the responsible molecular mechanism for phytoremediation to manage Cd soil contamination. var. is an ornamental perennial groundcover plant with strong tolerance to Cd. Previous studies found that , an AP2/ERF superfamily gene, may be an interacting partner of the metallothionein gene , which plays a key role in Cd tolerance. To study the role of in regulating Cd tolerance in , we analyzed its regulation function and mechanism based on a yeast two-hybrid assay, a bimolecular fluorescence complementation test, quantitative real-time PCR, transgenics and transcriptome sequencing. The results showed that IlAP2 interacts with IlMT2a and may cooperate with other transcription factors to regulate genes involved in signal transduction and plant hormones, leading to reduced Cd toxicity by hindering Cd transport. These findings provide insights into the mechanism of -mediated stress responses to Cd and important gene resources for improving plant stress tolerance in phytoremediation.
镉（Cd）胁迫对生态系统有重大影响，因此在阐明植物修复镉土壤污染的相关分子机制时，寻找合适的耐镉植物很重要。var. 是一种对镉具有较强耐受性的多年生观赏地被植物。先前的研究发现，作为AP2/ERF超家族基因的 ，可能是金属硫蛋白基因 的相互作用伙伴，该金属硫蛋白基因在镉耐受性中起关键作用。为了研究 在调节 镉耐受性中的作用，我们基于酵母双杂交试验、双分子荧光互补试验、定量实时PCR、转基因和转录组测序分析了其调控功能和机制。结果表明，IlAP2与IlMT2a相互作用，并可能与其他转录因子协同作用，调控参与信号转导和植物激素的基因，通过阻碍镉的转运降低镉毒性。这些发现为 介导的对镉胁迫反应的机制提供了见解，并为改善植物修复中植物胁迫耐受性提供了重要的基因资源。
